| Notch1胞内结构域在星形细胞肿瘤中的表达及其临床意义 |
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| 引用本文： | 杨东虹,唐文渊,徐伦山,肖华亮,周椿,许民辉. Notch1胞内结构域在星形细胞肿瘤中的表达及其临床意义[J]. 第三军医大学学报, 2010, 32(20) |
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| 作者姓名： | 杨东虹 唐文渊 徐伦山 肖华亮 周椿 许民辉 |
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| 作者单位： | 400016,重庆,重庆医科大学附属第一医院神经外科;400042,重庆,第三军医大学大坪医院野战外科研究所神经外科;重庆医科大学附属第一医院神经外科,重庆,400016;第三军医大学大坪医院野战外科研究所神经外科,重庆,400042;第三军医大学大坪医院野战外科研究所病理科,重庆,400042 |
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| 基金项目： | 国家自然科学基金面上项目(30700862,30872649)~~ |
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| 摘 要： | 目的探讨Notch1胞内结构域(intracellular Domain,ICD)在星形细胞肿瘤中的表达规律及其临床意义。方法收集大坪医院2009年1-6月间经手术切除的12例不同级别星形细胞肿瘤及2例瘤周正常脑组织新鲜标本,采用实时定量PCR方法检测Notch1ICD mRNA表达;收集85例有完整随访资料的星形细胞肿瘤存档标本,采用免疫组织化学技术检测Notch1ICD蛋白表达。结果 Notch1ICD在正常脑组织中低或不表达,肿瘤组织中Notch1ICD mRNA水平(4.65±3.04)显著高于正常脑组织(1.13±0.11)(P<0.01);高级别(WHOⅢ和Ⅳ级)星形细胞肿瘤Notch1ICD mRNA表达水平显著高于低级别星形细胞肿瘤(WHOⅡ级)[分别为(6.58±2.63)与(2.49±1.01),P<0.01]。免疫组化结果显示,相对于瘤周正常脑组织,各级星形细胞肿瘤Notch1ICD表达明显增强,并由单纯胞质表达向胞质胞核联合表达及单纯胞核表达过渡;Notch1ICD蛋白表达与组织学分级及患者的存活期有关,Notch1ICD高表达预示着较高的组织学分级和较短的存活时间。结论 Notch信...
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| 关 键 词： | 脑肿瘤 星形细胞 Notch 逆转录聚合酶链式反应 免疫组织化学 |
Expression of Notch1 intracellular domain in astrocytic tumors and their clinical significance:an analysis based on 97 cases |
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| Yang Donghong,Tang Wenyuan,Xu Lunshan,Xiao Hualiang,Zhou Chun,Xu Minhui. Expression of Notch1 intracellular domain in astrocytic tumors and their clinical significance:an analysis based on 97 cases[J]. Acta Academiae Medicinae Militaris Tertiae, 2010, 32(20) |
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| Authors: | Yang Donghong Tang Wenyuan Xu Lunshan Xiao Hualiang Zhou Chun Xu Minhui |
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| Affiliation: | Yang Donghong1,2,Tang Wenyuan1,Xu Lunshan2,Xiao Hualiang3,Zhou Chun2,Xu Minhui2(1Department of Neurosurgery,First Affiliated Hospital,Chongqing Medical University,Chongqing,400016,2Department of Neurosurgery,3Department of Pathology,Institute of Surgery Research,Daping Hospital,Third Military Medical University,400042,China) |
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| Abstract: | Objective To explore the expression pattern of Notch1 intracellular domain(ICD)in astrocytic tumors and its clinical significance.MethodsNotch1 ICD mRNA was detected in 12 fresh-resected astrocytic tumors of various grades after pathological diagnosis and 2 normal cerebral tissues in Daping Hospital from January to Jane in 2009 by real-time quantitative PCR.Notch1 ICD protein was detected in 85 archived astrocytic tumor samples at different grades using immunohistochemical method.ResultsMild or low expressi... |
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| Keywords: | brain tumor astrocyte Notch reverse transcriptase polymerase chain reaction immunohistochemistry |
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