3H11, a unique cell surface molecule involved in the function of the CD45RA+subset of CD4+ cells |
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Authors: | Hirose, Tatsuo Kobata, Tetsuji Nojima, Yoshihisa Schlossman, Stuart F. Morimoto, Chikao |
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Affiliation: | Division of Tumor immunology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School Boston, MA 02115. USA |
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Abstract: | We have developed a mAb anti-3H11 by immunizing mice with aT cell line derived from the Callithrix Jacchus (common marmoset).anti-3H11 is reactive with 48% of unfractlonated T cells, 62%of CD4+ cells and 39% of CD8+ cells. Among CD4 cells, anti-3H11preferentially reacts with the CD45RA+ T cell subset. The majorityof helper activity for pokeweed mitogen (PWM)-driven B cellIgG synthesis and T cell response to recall antigen such astetanus toxold was found within the 3H11-CD4 cell population,whereas anti-3H11+CD4+ cells provided poor helper function forPWM-driven B cell IgG synthesis and were more responsive toconcanavalln A and autologous mixed lymphocyte reaction. Biochemicalcharacterization showed that anti-3H11 precipitated a singleprotein band with a relative molecular weight of 32,000 from125l-surface labeled cell lysate. Biochemical, phenotyplc andfunctional studies revealed that the 3H11 molecule appearedto be different from previously established molecules on theT cell surface. Interestingly, addition of anti-3H11 to thecombination of CD4 and B cells in the presence of CDS cellsbut not to the combination of CD4 and B cells resulted in enhancementof the suppression of PWM-driven B cell IgG synthesis. Moreover,anti-3H11 had a co-mitogenic effect on T cells via the CD2 andCD3 pathways, and this co-mitogenic activity is restricted tothe CD45RA+ T cells. Taken together, our results show that the3H11 molecule is a novel antigen which may play an importantrole in the activation and function of the CD45RA+ subset ofT cells. |
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Keywords: | CD45RA+ T cells immunoregulation PWM-driven B cell IgG synthesis T cell co-stimulation |
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