幽门螺杆菌cagA菌株感染与胃癌组织中环氧化酶-Ⅱ表达的相关性研究

目的：探讨幽门螺杆菌cagA(Hp-cagA)菌株感染与胃癌组织中环氧化酶－Ⅱ（COX-2）表达之间的关系,为胃癌的早期预防提供有价值的实验和理论依据。方法：采用流式细胞术定量检测及分析31例胃癌组织及相应的癌旁正常胃组织中COX－2的表达水平,并通过PCR技术对胃癌组织中cagA基因进行扩增检测。结果：31例胃癌组织中,有26例COX－2过表达,相应的26例癌旁正常组织基本上无表达或弱表达。18例cagA阳性的胃癌组织中COX－2的表达水平明显高于13例cagA阴性的胃癌组织。结论：COX－2在胃癌组织中过度表达,cagA菌株感染可上调胃癌组织中COX-2绵表达水平。可能存在cagA菌株感染之外的其他因素或机制调节COX－2的表达。因此,在根治Hp感染的同时,应用COX－2特异性抑制剂可能是一种有效预防胃癌发生、发展的新路。

Association between Helicobacter pylori cagA strain infection and expression of cyclooxygenase 2 in gastric carcinoma

Objective To observe the expression of cyclooxygenase 2 (COX 2) in tissues of human gastric cancerr and explore the association between Helicobacter pylori cagA strain infection and the expression of COX 2 so as to provide a theoretical basis for early prevention of gastric cancer. Methods Flow cytometry was adopted to quantitatively determine and analyze the expression of COX 2 in 31 specimens of gastric cancer and normal juxta cancerous tissues. PCR was used to detect the cagA gene in gastric cancer. Results COX 2 was over expressed in 26 of the 31 (84%) specimens of gastric cancer. The expression of COX 2 in the 18 specimens of gastric cancer with cagA positive strain infection was significantly higher than that in the 13 specimens with cagA negative strain infection. Conclusion COX 2 is over expressed in gastric cancer and cagA positive strain infection up regulates the expression of COX 2 in gastric cancer. There may be another way or channel to regulate the expression of COX 2 in gastric cancer besides cagA positive strain infection. Therefore, applying COX 2 selective inhibitors to prevent gastric cancer can be an effective and promising way.