| Intrastriatal Gene Transfer of Vascular Endothelial Growth Factor Rescues Dopaminergic Neurons in a Rat Parkinson＇s Disease Model |
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| 引用本文： | 田有勇,孙圣刚,汤翠菊,王家宁,陈小武,乔娴.Intrastriatal Gene Transfer of Vascular Endothelial Growth Factor Rescues Dopaminergic Neurons in a Rat Parkinson＇s Disease Model[J].华中科技大学学报(医学英德文版),2006,26(6):670-673. |
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| 作者姓名： | 田有勇 孙圣刚 汤翠菊 王家宁 陈小武 乔娴 |
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| 作者单位： | [1]Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China [2]Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China [3]Institute of Clinical Medicine, the Affiliated People＇s Hospital of Yunyang Medical College, Shiyan 442000 China |
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| 摘 要： | To examine the ability of intrastriatal gene transfer of vascular endothelial growth factor 165 mediated by adenoviral vector to rescue dopaminergic neurons in a rat model of Parkinson＇s disease （PD）, we constructed recombinant replication-deficent adenoviral vectors carrying the gene of VEGF165 （Ad-VEGF）, and injected Ad-VEGF （or Ad-LacZ and PBS as controls） into the striatum of rats 7 days after the lesion by 6-hydroxydopamine. The rat rotational behavior analysis and tyrosine hydroxylase （TH） immunohistochemistry were performed to assess the change of dopaminergic neurons. Our results showed that the rats receiving Ad-VEGF injection displayed a significant improvement in apomorphine-induced rotational behavior and a significant preservation of TH-positive neurons and fibers compared with control animals, It is concluded that intrastriatal gene transfer by Ad-VEGF may rescue the dopaminergic neurons from degeneration in a rat model of PD.
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| 关 键 词： | 基因转换 血管内皮生长因子 多巴胺能 帕金森疾病 |
| 收稿时间： | 2006-08-20 |
| 修稿时间： | 2006-08-20 |
Intrastriatal gene transfer of vascular endothelial growth factor rescues dopaminergic neurons in a rat Parkinson’s disease model |
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| Youyong Tian M. D., Ph.D.,Shenggang Sun,Cuiju Tang,Jianing Wang,Xiaowu Chen,Xian Qiao.Intrastriatal gene transfer of vascular endothelial growth factor rescues dopaminergic neurons in a rat Parkinson’s disease model[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2006,26(6):670-673. |
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| Authors: | Youyong Tian M D PhD Shenggang Sun Cuiju Tang Jianing Wang Xiaowu Chen Xian Qiao |
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| Institution: | (1) Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;(2) Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China;(3) Institute of Clinical Medicine, the Affiliated People’s Hospital of Yunyang Medical College, Shiyan, 442000, China |
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| Abstract: | Summary To examine the ability of intrastriatal gene transfer of vascular endothelial growth factor 165 mediated by adenoviral vector
to rescue dopaminergic neurons in a rat model of Parkinson’s disease (PD), we constructed recombinant replication-deficent
adenoviral vectors carrying the gene of VEGF165 (Ad-VEGF), and injected Ad-VEGF (or Ad-LacZ and PBS as controls) into the
striatum of rats 7 days after the lesion by 6-hydroxydopamine. The rat rotational behavior analysis and tyrosine hydroxylase
(TH) immunohistochemistry were performed to assess the change of dopaminergic neurons. Our results showed that the rats receiving
Ad-VEGF injection displayed a significant improvement in apomorphine-induced rotational behavior and a significant preservation
of TH-positive neurons and fibers compared with control animals. It is concluded that intrastriatal gene transfer by Ad-VEGF
may rescue the dopaminergic neurons from degeneration in a rat model of PD. |
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| Keywords: | vascular endothelial growth factor A Parkinson's disease gene therapy gene transfer technique |
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