Production and Characterisation of Murine Monoclonal Antibodies to Feline Erythrocyte A and B Antigens |
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Authors: | J L Green P S Chavey G A Andrews J E Smith |
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Institution: | (1) Department of Diagnostic Medicine\Pathobiology, College of Veterinary Medicine, Kansas State University, Kansas, USA, US |
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Abstract: | Anti-A antiserum from blood type B cats, the current reagent used to detect blood type A cats, is expensive, labour intensive
to produce, and can vary in sensitivity between preparations. In contrast, monoclonal antibodies are produced easily in large
quantities and pure form. We produced six IgM class murine monoclonal antibodies, four specific for feline blood type A and
two that detect feline blood type B, by injection of mice with liposomes incorporating type A or B erythrocyte membrane antigens.
Specificities of each monoclonal antibody were characterised by high performance thin layer chromatography of feline erythrocyte
membrane glycolipids and by immunoblotting of feline erythrocyte membrane proteins separated by SDS-PAGE. The anti-A monoclonal
antibodies specifically detected feline blood type A by direct agglutination of blood-typed samples from many cats. Each anti-A
monoclonal antibody agglutinated some, but not all, feline blood type AB samples. Two anti-A monoclonal antibodies appeared
identical and recognised NeuGc]2GD3, the major glycolipid antigen of type A blood. The other two also appeared identical to each other and recognised a slower
migrating glycolipid band, which may be NeuGc]GT3. The two anti-B monoclonal antibodies detected feline blood type B by direct agglutination and both recognised NeuAc]2GD3, the major glycolipid antigen of type B blood. None of the monoclonal antibodies recognised erythrocyte membrane glycoproteins
specific for either feline type A or type B blood. The ability of the anti-A monoclonal antibodies produced in this study
to specifically detect feline blood type A makes them useful replacements for anti-A antiserum for blood typing of cats. The
inability of each anti-A antibody to agglutinate blood from every type AB cat suggests a difference between the A antigen
of some type A and some type AB cats. |
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Keywords: | :Blood type – Cat – Monoclonal antibody – Neonatal isoerythrolysis – Transfusion reaction |
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