PM2.5‐induced airway inflammation and hyperresponsiveness in NC/Nga mice |
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Authors: | Keiki Ogino Kenjiro Nagaoka Tomoaki Okuda Akira Oka Masayuki Kubo Eri Eguchi Yoshihisa Fujikura |
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Affiliation: | 1. Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Science, Kita‐ku, Okayama 700‐8558, Japan;2. Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan;3. Department of Molecular Anatomy, Faculty of Medicine, Oita University, Yufu, Oita 879-5593, Japan |
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Abstract: | The allergic inflammatory effects of particulate matter (PM) 2.5, collected with the cyclone system in Yokohama city in Japan, were investigated in NC/Nga mice, which are hypersensitive to mite allergens. PM2.5 with alum was injected intraperitoneally for sensitization. Five days later, 200 μg of PM2.5 in 25 μL of saline was administered to mice intranasally five times for further sensitization. On the 11th day, PM2.5 was administered as a challenge. On the 12th day, mice were examined for airway hyperresponsiveness (AHR), the bronchoalveolar lavage fluid (BALF) cell count, mRNA expression of Th1, Th2 cytokines, and metallothioneins in lung tissue, and histopathology. PM2.5 increased AHR, total cell numbers including eosinophils in BALF, and mRNA levels of IL‐5, IL‐22, eotaxin, eotaxin 2, and metallothionein 3. In PM2.5‐induced lungs, inflammation was observed around the bronchus. These results demonstrate that PM2.5 alone, collected with the cyclone system in Yokohama city in Japan, induces asthma‐like airway inflammation. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1047–1054, 2017. |
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Keywords: | allergic airway inflammation PM2.5 NC/Nga mice |
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