Duchesnea indica extract suppresses the migration of human lung adenocarcinoma cells by inhibiting epithelial–mesenchymal transition |
| |
| Authors: | Pei‐Ni Chen Shun‐Fa Yang Cheng‐Chia Yu Chin‐Yin Lin Shih‐Han Huang Shu‐Chen Chu Yih‐Shou Hsieh |
| |
| Affiliation: | 1. Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan;2. Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan;3. Medicine, Chung Shan Medical University, Taichung, Taiwan;4. Institute of Oral Science, School of Dentistry, Chung Shan Medical University, Taichung, Taiwan;5. Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan;6. Institute and Department of Food Science, Central Taiwan University of Science and Technology, Taichung, Taiwan |
| |
| Abstract: | Epithelial–mesenchymal transition (EMT) is a process through which epithelial cells are transformed into mesenchymal cells; EMT diminishes cell polarity and cell–cell adhesion in cancer cells, leading to enhanced migratory and invasive properties. In this experiment, zymography, cell invasion, and migration assays were performed. Results indicated that Duchesnea indica extracts (DIE) inhibited highly metastatic A549 and H1299 cells by reducing the secretions of matrix metalloproteinase‐2 and urokinase‐type plasminogen activator. Cell adhesion assay also demonstrated that DIE reduced the cell adhesion properties. Western blot analysis showed that DIE down‐regulated the expression of N‐cadherin, fibronectin, and vimentin, which are mesenchymal markers, and enhanced that of E‐cadherin, which is an epithelial marker. In vivo study showed that tumor growth was significantly reduced in BALB/c nude mouse xenograft model administered with oral gavage of DIE. Therefore, DIE could be exhibits potential as a phytochemical‐based platform for prevention and treatment of lung cancer. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 2053–2063, 2017. |
| |
| Keywords: | Duchesnea indica epithelial– mesenchymal transition lung adenocarcinoma matrix metalloproteinase metastasis urokinase‐type plasminogen activator |
|
|
