Biocompatibility assessment of nanomaterials for environmental safety screening |
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| Authors: | Yi‐Hui Lee Show‐Mei Chuang Sheng‐Chi Huang Xiaotong Tan Ruei‐Yue Liang Gordon C C Yang Pin Ju Chueh |
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| Institution: | 1. Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan;2. Institute of Environmental Engineering and Center for Emerging Contaminants Research, National Sun Yat‐Sen University, Kaohsiung, Taiwan;3. Graduate Institute of Basic Medicine, China Medical University, Taichung, Taiwan;4. Department of Medical Research, China Medical University Hospital, Taichung, Taiwan;5. Department of Biotechnology, Asia University, Taichung, Taiwan |
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| Abstract: | In view of the extensive use of nanoparticles in countless applications, a fast and effective method for assessing their potential adverse effects on the environment and human health is extremely important. At present, in vitro cell‐based assays are the standard approach for screening chemicals for cytotoxicity because of their relative simplicity, sensitivity, and cost‐effectiveness compared with animal studies. Regrettably, such cell‐based viability assays encounter limitations when applied to determining the biological toxicity of nanomaterials, which often interact with assay components and produce unreliable outcomes. We have established a cell‐impedance‐based, label‐free, real‐time cell‐monitoring platform suitable for use in a variety of mammalian cell lines that displays results as cell index values. In addition to this real‐time screening platform, other traditional cytotoxicity assays were employed to validate cytotoxicity assessments. We suggest that the cell impedance measurement approach is effective and better suited to determining the cytotoxicity of nanomaterials for environmental safety screening. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1170–1182, 2017. |
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| Keywords: | cytotoxicity cell‐impedance measurement nanomaterials in vitro assays |
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