藏医佐木阿汤与放血疗法保护HAPC大鼠心肌的形态学研究

目的：观察藏医佐木阿汤及放血疗法对高原红细胞增多症（HAPC）模型大鼠心肌形态的影响。方法：选用雄性wistar大鼠120只，随机分为3，6，9，12月组4组，再随机分为高原模型组、平原对照组、药物组、放血组、药物加放血组。除平原对照组外，余组均饲养于拉萨以建立HAPC模型。饲养3个月后开始干预，分别于3，6，9，12个月时切取心肌组织进行光镜和电镜观察。结果：光镜下，各月组干预后，药物组、放血组、药物加放血组心肌损伤程度较高原对照组表现为轻，同时药物加放血组心肌损伤程度较药物和放血组表现略重。干预三组电镜下的心肌损伤程度较高原对照组表现轻，三组间损伤程度差异不显著。结论：佐木阿汤和放血疗法对HAPC大鼠心肌有一定的保护作用。

Morphological study of using traditional Tibetan medicine Zuomua decoction and blood-letting therapy to protect myocard of HAPC rats

Objective ：To observe morphological change in high altitude polycythemia（HAPC） rats＇ myocard treated by tradi- tional Tibetan medicine Zuomua decoction and blood-letting therapy. Methods：Total 120 male Wistar rats were selected and randomly divided into 4 groups,which include 3,6,9,12 month groups. Then,we assign each group into model control group, normal control group,decoction group,blood-letting therapy group,decoction combined with blood-letting therapy group. Each group was fed in Lhasa to establish HAPC model except normal control group. After 3 months,the models were treated with drugs and blood-letting therapy separately and myocardial tissues were observed at 3rd,6th,9th, 12th month by light microscope and electron microscope separately. Results：The light microscope showed that the injury degree of myocardium in decoction group,blood-letting therapy group,decoction combined with blood-letting therapy group were slighter than that in model control group. The injury degree of myocardium in decoction combined with blood-letting therapy group was severer than that in de- coction group,blood-letting therapy group. The electron microscope showed that the injury degree of myocardium in three treat- ment groups was slighter than model control group and there were not significant differences between three groups. Conclusion： Zuomua decoction and blood-letting therapy have some effect of protecting myocard of HAPC rats.