大豆异黄酮对去势大鼠骨量丢失的抑制作用研究

目的:研究大豆异黄酮(Soybeanisoflavone,SI)对去势后大鼠由于体内雌激素水平下降引起的骨量丢失的抑制作用。方法:腹腔手术切除大鼠双侧卵巢,分为对照组、SI组和雌激素对照组,并设假手术对照组,喂养16w后,测定雌二醇、骨生化指标、骨密度、骨钙、骨磷等指标,扫描电镜和组化分析观察骨小梁微观结构的变化。结果:SI显著降低血清中骨吸收指标TRAP活性,同时明显提高骨形成指标BGP含量;SI具有弱雌激素作用,可抑制去卵巢大鼠体内雌激素水平的下降,骨密度和骨钙、骨磷含量增加,松质骨的骨丢失得到抑制。结论:SI可降低骨吸收、促进骨形成,对去卵巢引起的骨量丢失有明显的抑制作用。

STUDY ON THE INHIBITIVE EFFECT OF BONE LOSS BY SOYBEAN ISOFLAVONE IN OVARIECTOMIZED RATS

Objective: To study the inhibitive effect of soybean isoflavone on bone loss induced by the decrease of estrogen level in ovariectomized rats. Methods: Ovariectomized Wistar rats (n=48) were divided into six groups and given basic diet, or containing soybean isoflavone or diethylstilbestrol diet. After 16 weeks,estrogen and TRAP (tartrate -resistant acid phosphatase) and BGP (bone Gla-containing protein) in serum were determined. BMD (bone mineral density), bone calcium and phosphorus were measured. By using scanning electron microscope and histochemistry methods to observe the change of microstructure in trabecular bone. Result: Soybean isoflavone can significantly decrease the activity of bone resorption marker TRAP and increase the content of bone formation marker BGP. Soybean isoflavone posses weak estrogen effect and increase femur BMD and Ca, P content. Conclusion:Soybean isoflavone have significantly effect of anti-bone loss in ovariectomized rats.