加减补肺汤联合甲强龙对IPF大鼠抗氧化作用的影响

目的:观察加减补肺汤联合甲强龙对博来霉素诱导的特发性肺纤维化(IPF)大鼠抗氧化作用,探讨其作用机制。方法:60只SD大鼠随机分为正常组,模型组,甲强龙组(10 mg·kg~(-1)·d~(-1)),加减补肺汤组(100 mg·kg~(-1)·d~(-1)),联合用药1组,联合用药2组,每组10只。除正常组外其余各组大鼠采用气管内一次性滴注博来霉素复制IPF模型(1 mg/只),造模24 h后,开始ig或im给药,持续到处死动物的前1 d,模型组ig等体积的生理盐水。计算IPF大鼠肺系数,观察肺组织形态学改变,测定各组大鼠肺组织匀浆中羟脯氨酸(Hyp),丙二醛(MDA)含量和超氧化物歧化酶(SOD)的活性,取固定部位肺组织分别进行苏木素-伊红(HE)染色。结果:与正常组比较,模型组大鼠肺泡炎细胞浸润及肺间质纤维化程度明显,肺系数明显升高,肺组织中Hyp,MDA含量明显升高、肺组织中SOD的活性明显降低(P0.05,P0.01);与模型组比较,各给药组均可改善IPF大鼠的生存状态,甲强龙组、加减补肺汤组和联合用药1组可明显改善IPF大鼠肺泡炎细胞浸润及肺间质纤维化程度,显著降低肺系数(P0.01,P0.05),降低肺组织中Hyp,MDA含量、升高肺组织中SOD的活性。结论:联合用药1组对降低IPF大鼠肺组织中肺系数,Hyp和MDA的含量,升高肺组织中SOD活性,优于单独给药,其机制可能是减少脂质过氧化物的释放而达到抗氧化作用。

Antioxidant Effects of Modified Bufei Tang Combined with Methylprednisolone on IPF Rats

Objective: To observe the antioxidant effects of modified Bufei Tang (MBT) combined with methylprednisolone on bleomycin-induced idiopathic pulmonary fibrosis (IPF) in rats, and investigate the mechanism. Method: Sixty Sprague-Dawley rats were randomly divided into normal group, model group, methylprednisolone group (10 mg·kg^-1·d^-1), MBT group (100 mg·kg^-1·d^-1), combined treatment group 1, and combined treatment group 2, n=10 in each group. The IPF models were reproduced by intratracheal injection of bleomycin (1 mg for each rat). 24 hours after modeling, the rats were treated with daily intragastric administration or intramuscular injection until one day before sacrificing. The rats in model group were treated with the same volume of normal saline. Lung coefficient was calculated, and lung tissue morphology changes were observed in IPF rats. The hydroxyproline (Hyp), malondialdehyde (MDA) and superoxide dismutase (SOD) levels in lung homogenates of rats were measured. Hematoxylin-eosin staining was applied for fixed lung tissues. Result: As compared with the normal group, degree of alveolar inflammatory cells infiltration and pulmonary fibrosis was obvious;lung coefficient was increased significantly;Hyp and MDA levels in liver tissues were increased significantly;and SOD activity in liver tissues was decreased significantly in the rats of model group (P<0.05, P<0.01). Various treatment groups could improve the living state of IPF rats;methylprednisolone group, MBT group, and combined treatment group 1 could significantly improve the degree of alveolar inflammatory cells infiltration and pulmonary interstitial fibrosis of IPF rats, significantly reduce lung coefficient (P<0.01, P<0.05), reduce Hyp and MDA levels in the lung tissues and increase SOD activity in the lung tissues. Conclusion: Combined treatment group 1 has superior effect than single treatment in reducing liver coefficient, Hyp and MDA levels and increasing SOD activity in the lung tissues of IPF rats. The mechanism might be related to decreasing the level of lipid peroxide to achieve antioxidant effects.