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| 乌药茎中鞣质类成分及其抗HIV-1整合酶活性研究 | |
| 引用本文： | 张朝凤,孙启时,王峥涛,服部征雄. 乌药茎中鞣质类成分及其抗HIV-1整合酶活性研究[J]. 中国药学杂志, 2003, 38(12): 911-914 |
| 作者姓名： | 张朝凤 孙启时 王峥涛 服部征雄 |
| 作者单位： | 1. 中国药科大学生药研究室,南京,210038 2. 沈阳药科大学药用植物教研室,沈阳,110016 3. 富山医科药科大学和汉药研究所药物代谢工学,富山,930-0194,日本 |
| 摘 要： | 目的研究樟科药用植物乌药［Lindera aggregata(Sims) Kosterm.］的抗HIV-1整合酶的活性成分。方法采用60%丙酮冷浸提取，通过反复Sephadex LH-20和MCI-gel CHP 20P柱色谱从乌药茎中分离缩合鞣质类化合物，通过波谱解析和理化常数进行结构鉴定，采用MIA法进行抗HIV-1整合酶活性筛选。结果分离得到3个寡聚缩合鞣质类化合物：二倍体procyanidin B-1(LA-1),三倍体cinnamtanin B1(LA-2)和四倍体cinnamtannin B2(LA-3)。活性筛选结果表明，寡聚缩合鞣质具有抗HIV-1整合酶的活性。结论寡聚缩合鞣质是乌药茎抗HIV-1整合酶的主要活性成分，四倍体cinnamtannin B2(3)为种内首次分离得到。 |
| 关 键 词： | 乌药 缩合鞣质 HIV-1整合酶 |
| 文章编号： | 1001-2494(2003)12-0911-04 |
| 收稿时间： | 2003-01-02; |
| 修稿时间： | 2003-01-02 |
Inhibitory activities of tannins extracted from stem of Lindera aggregata against HIV-1 integrase |
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| Tewtrakul Supinya. Inhibitory activities of tannins extracted from stem of Lindera aggregata against HIV-1 integrase[J]. Chinese Pharmaceutical Journal, 2003, 38(12): 911-914 | |
| Authors: | Tewtrakul Supinya |
| Affiliation: | 1.College of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 210038,China;2.College of Traditional Chinese Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;3.Research Institute for Wakan yaku,Toyama Medical and Pharmaceutical University,Toyama 930-0194,Japan |
| Abstract: | OBJECTIVETo obtain compounds with inhibitory act iv ities against HIV-1 integrase from the stem of Lindera aggregata (Sims) Kos term..METHODSThe compounds were extracted with 60% acetone, isolated via column chromatography on Sephadex LH-20 and MCI-gel CHP-20P.The inhibitory activities were studied by the method of MIA(multiplate integration a ssay).RESULTSThree oligomeric procyanidins were isolated fr om the stem of Lindera aggregata.On the basis of the properties and spectral analysis,their structures were identified as: procyanidin B 1(LA-1),cinnamtan in B 1(LA-2) and cinnamtannin B 2(LA-3).MIA test showed the three compounds had inhibitory activities against HIV-1 integrase.CONCLUSION Oligomeric procyanidins may be the main active components of the stem of Lin dera aggregata against HIV-1 integrase.Cinnamtannin B 2(LA-3) was isolated from this plant for the first time. |
| Keywords: | Lindera aggregata condensed tannin identificat ion HIV-1 integrase |
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