双波长法快速测定蛋白质含量

目的　为了建立一种快速而灵敏的蛋白质检测方法。方法　在考马斯亮兰法测定蛋白质含量时，通过采用５９０ｎｍ 和４５０ｎｍ 双波长代替单波长检测，并以酶标检测仪取代紫外－ 可见分光光度计作为检测仪。结果　此种改进不但提高了考马斯亮兰法检测蛋白质的灵敏度（０ ．１２７μｇ／ｍｌ） 和检测范围（０ ．１２７ ～３１ ．２５μｇ／ｍｌ），而且使大量标本的检测可在一次检测过程中完成。标准直线相关系数ｒ２ ＝ ０ ．９９６５，表明在检测限内Ａ５９５／Ａ４５０ 和蛋白质浓度Ｃ呈良好线形关系。结论　双波长法可作为一种快速、灵敏的蛋白质检测方法。

Rapid Two-colorimetric Assay for Protein Quantities

Objective To improve the sensitivity of the Coomassie brilliant blue protein assay.Methods The Coomassie brilliant blue assay is accomplished by measurement of absorbance at 590 and 450nm.To linear with protein concentration by the ratio of the absorbance,590 nm over 450 nm,but not the absorbance of 590nm,and replace the UV/VIS spectrophotometer with the microplate autoreader as the detecting equipment.Results This simple process increases the accuracy and improves the sensitivity of the assay about 20-fold,also make a lot of samples be detected in one cycle.Conclusions This assay can be as sensitive and rapid a method for protein quantities.