C型肉毒梭菌毒素冻干剂对高原鼠兔的灭效试验

目的试验100万毒价／ml C型肉毒梭菌毒素冻千剂对高原鼠兔的杀灭效果。方法采用田间试验，共设试验小区5个，小区向积均为3hm^2。其中0．1％和0．15％浓度小麦毒饵各投放2个小区，另一小区作为对照投放0．1％水剂小麦毒饵。此外，设一面积为0．25hm^2的空白对照区，以测定自然灭洞率。结果灭前试验区高原鼠兔平均密度为1071．4洞口／hm^2。投饵后第6天浓度为0．1％的小麦毒饵平均校正灭洞率为96．7％，浓度为0．15％的毒饵平均校正灭洞率为97．4％。处理对照区的平均校正灭洞率为97．7％；各浓度间校正灭洞率差异无统计学意义。试验期间，在试验地及其周围500m地区未发现非靶标动物中毒死亡现象。但在长时间及大范围内，该药剂的安全性需更进一步的验证。结论肉毒梭菌毒素冻干剂与同效价水剂差异无统计学意义。

Study on the efficiency of one million MLD/ml Type-C Botulinum lyophilized preparation to Killing Plateau pika (Ochotona curzoniae)

Objective To study the killing efficiency of one million MLD/ml Type-C Botulinum lyophilized preparation to Ochotona curzoniae. Methods Five plots was selected to test and each area was 3 hm^2 . 0.1% wheat baits were put in two plots randomly, other two-plot for 0.15 % and the last one for aqueous agent. Besides, the control was 0.25 hm^2. Results The density of O. curzoniae was 1071.4 holes/hm^2 before treatment. The killing efficiency of 0.1% wheat baits and 0.15% wheat baits were 96.7% and 97.4% after treatment for six days, respectively. However, the killing efficiency was also 97.7% in the control plots. It showed that there was no significant difference among them. During the test, there were no no-target animals to be poisoned in test plots and within 500 m of it. It indicated that Type-C Botulinum was safe to some degree, but the safety of the chemicals was still further validated in the long run. Conclusion There is no significant difference between botulinum lyophilized preparation and the same titer aqueous botulinum.