凤尾草总黄酮对肺癌细胞迁移抑制作用及机制的研究＊

目的 分析凤尾草总黄酮对人肺癌A549细胞迁移能力的影响,并探索其中的机制。方法 将不同浓度的凤尾草总黄酮与人肺癌A549细胞常规培养,并分别进行细胞划痕实验和Transwell实验,观察肿瘤细胞迁移能力与凤尾草总黄酮浓度之间的关系。利用Westen blot方法检测A549细胞内成纤维生长因子4（FGF4）蛋白表达水平与凤尾草总黄酮浓度之间的关系。结果 实验组划痕间距离缩短程度、细胞过胞膜数量和FGF4蛋白表达水平与对照组相比有所降低,并且随着加入凤尾草总黄酮浓度的升高而降低越明显。结论 凤尾草总黄酮可抑制肺A549细胞的迁移,其发生机制可能与肿瘤细胞内FGF4蛋白表达水平下降有关。

Inhibitory Effect of Total Flavonoids from Pteris Multifida on Lung Cancer Cell Migration and Its Mechanism

Objective To analysis the effect of the Pteris multifida Flavones on A549 cells metastasis of human lung cancer cell line in vitro. So to explore the action mechanisms of the Pteris multifida Flavones.Methods Adding different concentration of total flavonoids bracken to A549 lung cancer cell culture medium by cell scratch test, Transwell metastasis experiments, and observe the metastasis ability of A549 cells. The levels of FGF4 protein in A549 cells was detected by Western Blotting.Results The experimental group inhibited the A549 cells wound closure significantly and the number of cells passing through filter membranethe. And levels of FGF4 protein in A549 cells were decreased significantly comparing with control group after treated with the Pteris multifida Flavones.Conclusion The Pteris multifida Flavones is able to inhibit the metastasis ability of A549 cells by inhibition of FGF4 protein levels in A549 cells.