Investigation of the influence of calibration practices on cytogenetic laboratory performance for dose estimation |
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Authors: | François Trompier Marion Baumann Lleonard Barrios Eric Gregoire Michael Abend Elizabeth Ainsbury |
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Institution: | 1. Institut de Radioprotection et de S?reté Nucléaire (IRSN), Fontenay-aux-roses, France;2. francois.trompier@irsn.fr;4. Universitat Autònoma de Barcelona, Bellaterra, Spain;5. Bundeswehr Institut für Radiologie in verbindung mit der Universtit?t Ulm, Germany;6. Public Health England Centre for Radiation, Chemical and Environmental Hazards (PHE), Chilton, UK |
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Abstract: | AbstractPurpose: In the frame of the QA program of RENEB, an inter-laboratory comparison (ILC) of calibration sources used in biological dosimetry was achieved to investigate the influence of calibration practices and protocols on the results of the dose estimation performance as a first step to harmonization and standardization of dosimetry and irradiation practices in the European biological dosimetry network.Materials and methods: Delivered doses by irradiation facilities used by RENEB partners were determined with EPR/alanine dosimetry system. Dosimeters were irradiated in the same conditions as blood samples. A short survey was also performed to collect the information needed for the data analysis and evaluate the diversity of practices.Results: For most of partners the deviation of delivered dose from the targeted dose remains below 10%. Deviations larger than 10% were observed for five facilities out of 21. Origins of the largest discrepancies were identified. Correction actions were evaluated as satisfactory. The re-evaluation of some ILC results for the fluorescence in situ hybridization (FISH) and premature chromosome condensation (PCC) assays has been performed leading to an improvement of the overall performances.Conclusions: This work has shown the importance of dosimetry in radiobiology studies and the needs of harmonization, standardization in irradiation and dosimetry practices and educational training for biologists using ionizing radiation. |
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Keywords: | Alanine dosimetry biological dosimetry calibration inter-laboratory comparison quality assurance |
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