恒河猴感染戊型肝炎病毒的保护性研究

该文报告恒河猴感染戊型肝炎病毒(HEV)后数月再次攻击HEV的保护性研究。实验组4只猴在第1次静脉注射HEV后25～30天,均出现了典型的ALT升高。肝组织呈现血型的急性肝炎特征,急性期粪便中检查到典型的27～34nm的病毒颗粒。对照组3只猴静脉注射一种致细胞病变的肠道病毒后未发生ALT升高。6个月后,上述7只猴一起攻击同等剂量、同样来源的HEV,并经过1个多月的观察。实验组4只猴全部未发生ALT升高,而对照组3只猴分别在攻击HEV后24、26和26天出现了典型的ALT升高,ALT升高的潜伏期和升高的持续时间与实验组4只猴第1次感染HEV的ALT升高情况完全一致。免疫电镜结果表明,实验组4只猴在第1次感染HEV后ALT升高期间以及恢复正常后2～3个月内,血清中存在集聚HEV的抗体。

PROTECTION OF RHESUS MONKEYS FROM CHALLENGE OF HEPATITIS E VIRUS

Protection of Rhesus monkeys from challenge of hepatitis E virus (HEV) 6 months after the first inoculation was studied. There were 4 monkeys in experimental group who had developed typical elevation of liver enzyme values and characteristic histopathologic changes associated with acute viral hepatitis and excretion of 27 to 34 nm virus-like particles and anti-HEV antibodies within 3 months after the inoculation with 2 ml of 10% stool or liver suspensions from the HEV-infected monkeys. There were 3 monkeys in control group who had not developed any changes associated with viral hepatitis after the inoculation with a strain of enteric virus in cell cultures. 7 Monkeys were inoculated with 2 ml of 10% Stool suspension from the HEV-infected monkeys 6 months after the first inoculation. 3 Monkeys in control group developed significant ALT elevation and histopathologic changes of viral hepatitis at 24, 26, and 26 days after challenge of HEV and 4 monkeys in experimental group did not develop any changes.