| 两种检测方法对EV71病毒监测结果的影响研究 |
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| 引用本文： | 梁英,怀清杰,张炳丽,梁爽,任莉娜,范荣军,李晓鹏. 两种检测方法对EV71病毒监测结果的影响研究[J]. 中国卫生检验杂志, 2010, 0(7) |
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| 作者姓名： | 梁英 怀清杰 张炳丽 梁爽 任莉娜 范荣军 李晓鹏 |
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| 作者单位： | 哈尔滨市疾病预防控制中心; |
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| 基金项目： | 哈尔滨市科技创新人才研究专项资金项目(2009RFXQS008) |
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| 摘 要： | 目的:比较RT-PCR法与RD细胞病毒分离法两种检测方法对监测EV71病毒结果的影响。方法:将151份标本分别经RT-PCR法与RD细胞病毒分离法检测出的阳性标本按不同分类及标本总阳性率进行数据统计,比较两种检验方法间的差异。结果:RT-PCR法与RD细胞病毒分离法检测出的阳性标本按不同分类统计比较后,两者无明显差异,无统计学意义(P0.05);两种方法检测出的标本总阳性率RD细胞病毒分离法为38.41%,而RT-PCR法为26.49%,差异有统计学意义(2χ=4.89,P(0.05)。结论:RT-PCR法检测结果可以作为手足口EV71病毒监测结果进行分类数据统计分析,而标本监测年度总阳性率应以RD细胞病毒分离检测结果统计。
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| 关 键 词： | RT-PCR法 RD细胞病毒分离法 肠道病毒71型 监测结果 对比研究 |
Investigations on influence of two methods on detection of EV71 virus |
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| LIANG Ying,HUAI Qing-jie,ZHANG Bing-li,LIANG Shuang,REN Li-na,FAN Rong-jun,LI Xiao-peng. Investigations on influence of two methods on detection of EV71 virus[J]. Chinses Journal of Health Laboratory Technology, 2010, 0(7) |
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| Authors: | LIANG Ying HUAI Qing-jie ZHANG Bing-li LIANG Shuang REN Li-na FAN Rong-jun LI Xiao-peng |
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| Affiliation: | LIANG Ying,HUAI Qing-jie,ZHANG Bing-li,LIANG Shuang,REN Li-na,FAN Rong-jun,LI Xiao-peng (Harbin Center for Disease Control and Prevention,Harbin 150056,China) |
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| Abstract: | Objective:To compare the effects of the method of RT-PCR and RD cell-virus separation on detection of EV71 virus.Methods:Positive samples were sorted out with the method of RT-PCR and RD cell-virus separation from 151 samples,respectively.The statistic analysis was established in different classifications and total positive rates were also compared.The difference between the two methods were compared.Results:Comparing the statistics of the two different methods in different classifications,RD cell-virus sep... |
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| Keywords: | RT-PCR method RD cell-virus separation method EV71 virus Detection Comparison study |
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