| Nm23-H1基因沉默后K562细胞中肿瘤相关基因的差异表达 |
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| 引用本文： | 刘慧,金琳,张传海,熊盛,刘秋英,王一飞.Nm23-H1基因沉默后K562细胞中肿瘤相关基因的差异表达[J].中国卫生检验杂志,2010(5). |
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| 作者姓名： | 刘慧 金琳 张传海 熊盛 刘秋英 王一飞 |
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| 作者单位： | 暨南大学生物医药研究开发基地;皖西学院; |
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| 基金项目： | 国家高技术研究发展计划(863计划)资助课题(2007AA02Z142) |
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| 摘 要： | 目的:应用基因芯片技术,检测nm23-H1基因沉默后K562细胞中与肿瘤相关基因的差异表达情况,探讨nm23-H1基因沉默对K562细胞生物学行为变化的分子作用机制。方法:采用Trizol一步法抽提K562-siNM23细胞和K562-siNC细胞总RNA;用RNA扩增试剂盒进行双链cDNA的合成及荧光标记cRNA的合成,并纯化cRNA。与功能分类基因芯片杂交反应后,用计算机分析比较2种细胞中与肿瘤相关基因的差异性表达。结果:共筛选出表达差异性基因14个,其中表达上调基因9个(ratio2);表达下调基因5个(ratio0.5)。结论:nm23-H1基因沉默后,引起显著变化的基因与细胞周期调控和抗凋亡信号转导等生物学功能相关。
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| 关 键 词： | nm23-H1基因 功能分类基因芯片 差异表达 |
Differential expression of tumor-associated genes in K562 cells with nm23-H1 gene silencing |
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| LIU Hui,JIN Lin,ZHANG Chuan-hai,XIONG Sheng,LIU Qiu-ying,WANG Yi-fei.Differential expression of tumor-associated genes in K562 cells with nm23-H1 gene silencing[J].Chinses Journal of Health Laboratory Technology,2010(5). |
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| Authors: | LIU Hui JIN Lin ZHANG Chuan-hai XIONG Sheng LIU Qiu-ying WANG Yi-fei |
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| Institution: | LIU Hui1,JIN Lin1,ZHANG Chuan-hai2,XIONG Sheng1,LIU Qiu-ying1,WANG Yi-fei1 |
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| Abstract: | Objective:To explore differential expression panel of tumor-associated genes and the molecular mechanism of biology behavioral change in nm23-H1 gene silencing K562 cells.Methods:Total cellular RNA of K562-siNM23 and K562-siNC cells were isolated by one-step TRIzol extraction and reversely transcribed to the cDNA.Fluorescently labeled cRNA was synthesized by RNA amplification kit and purified by cRNA purification kit and then hybridized to the functional classification microarray.Hybridization signal was co... |
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| Keywords: | nm23-HI gene Functional classification microarray Differential expression |
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