| 不同理化因子对绞股蓝毛状根诱导的影响 |
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| 引用本文： | 何凤发,田恬,王贵学,唐茜,张建佳.不同理化因子对绞股蓝毛状根诱导的影响[J].中药材,2010(11). |
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| 作者姓名： | 何凤发 田恬 王贵学 唐茜 张建佳 |
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| 作者单位： | 西南大学农学与生物科技学院;重庆大学生物工程学院; |
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| 摘 要： | 目的:利用发根农杆菌诱导绞股蓝,得到毛状根。方法:采用外植体共感染接种法诱导绞股蓝毛状根,研究了不同菌株、外植体类型、预(共)培养时间、菌液浓度、浸染时间、乙酰丁香酮(As)和除菌培养基类型等因素对转化率的影响。结果:利用A4菌浸染不经过预培养的叶片,当发根农杆菌浓度在OD 600值为0.8,浸染10 min,共培养2 d,100μmol/L As、MS+300 mg/L Cab为除菌培养基为绞股蓝毛状根诱导的最佳条件。结论:用外植体共感染法诱导出绞股蓝毛状根,并确定了最佳诱导条件。
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| 关 键 词： | 绞股蓝 毛状根 诱导 |
Effects of Different Factors on Transformed Hairy Root in Gynostemm apentaphyllum |
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| HE Feng-fa,TIAN Tian,WANG Gui-xue,TANG Qian,ZHANG Jian-jia.Effects of Different Factors on Transformed Hairy Root in Gynostemm apentaphyllum[J].Jorunal of Chinese Medicinal Materials,2010(11). |
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| Authors: | HE Feng-fa TIAN Tian WANG Gui-xue TANG Qian ZHANG Jian-jia |
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| Institution: | HE Feng-fa1,2,TIAN Tian1,WANG Gui-xue2,TANG Qian1,ZHANG Jian-jia1(1.College of Agronomy and Biotechnology,Southwest University,Chongqing 400716,China,2.Bioengineering College,Chongqing University,Chongqing 400044,China) |
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| Abstract: | Objective:To induce hairy roots of Gynostemm apentaphyllum by Agrobacterium rhizogenes strains.Methods: Hairy roots were induced by the co-culture method of explants and Agrobacterium rhizogenes strains.Effects of different Agrobacterium rhizogenes strains,explants,pre(co)-culture time,Bacterial concentration,infecting time,As concentration and antibiotic medium on the transformation frequency were studied.Results: The highest induction frequency was obtained form leaf 2 days co-cultivation,which were induc... |
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| Keywords: | Gynostemm apentaphyllum(Thunb) Makino Hairy roots Induction |
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