组织因子途径抑制物-2对胰腺癌细胞增殖的影响

目的：检测人胰腺癌组织中组织因子途径抑制物-2（TFPI-2）基因的表达,探讨其对胰腺癌细胞增殖的影响.方法：采用免疫组织化学方法检测43例胰腺癌及9例正常胰腺组织中TFPI-2及Ki-67蛋白的表达.结果：胰腺癌中TFPI-2的表达低于正常胰腺组织,其表达水平与胰腺癌临床分期及转移有关, 于Ⅰ、Ⅱ期及无转移的胰腺癌组织中的表达高于Ⅲ、Ⅳ期及有转移的胰腺癌组织（P＜0.05）.其表达水平与胰腺癌细胞增殖活性呈负相关（rs=- 0.339,P＜0.05）.结论：TFPI-2基因在胰腺癌中呈低表达,能抑制肿瘤细胞的增殖,可能参与了胰腺癌细胞周期的调控.

Inhibitory effect of tissue factor pathway inhibitor-2 on cell proliferation of pancreatic carcinoma

Objective To detect the expression of tissue factor pathway inhibitor-2 （TFPI-2） in pancreatic carcinoma and to explore its effect on cell proliferation. Methods The expressions of TFPI-2 and Ki-67 were detected in 43 samples of pancreatic carcinoma and 9 normal pancreatic tissues using immunohistochemistry. Results The level of TFPI-2 expression in pancreatic carcinoma was lower than that in normal pancreatic tissues and was related to clinical stage and metastasis. TFPI-2 expression in the cancerous tissues from stage Ⅰ or Ⅱ patients or patients without metastasis was higher than that in the tissues from stage Ⅲ or Ⅳ patients or patients with metastasis （P 〈 0.05）. The level of TFPI-2 expression was negatively related with the activity of cancerous cell proliferation （rs=-0.339,P 〈 0.05）. Conclusion The low expression of TFPI-2 in pancreatic carcinoma can inhibit the proliferation of the cancerous cells, which may be involved in regulation of the cell cycle.