Wg3h细胞系转染PSV_2-neo质粒前后生长特性及表面超微结构改变的研究

本文对转染PSV_2-neo质粒后的Wg3h细胞系(Wg3h-neo)在长期传代中的生长特性和表面超微结构与母系Wg3h细胞进行了比较研究。结果发现:转染后Wg3h细胞的DNA合成及生长速度明显高于母系Wg3h细胞,生长饱和密度增大。在软琼脂培养中不形成集落,接种在裸鼠不长肿瘤。在扫描电镜下,细胞表面的微绒毛较母系Wg3h细胞丰富。Southern印迹杂交实验证明PSV_2-neo质粒已整合到宿主细胞的基因组中。转染后Wg3h细胞的生长特性和表面超微结构均发生了某些转化特征。

A STUDY ON THE CHANGES OF GROWTH CHARACTERISTICS AND SURFACE ULTRASTRUCTURE OF MG3H BEFORE AND AFTER TRANSFECTION WITH PSV2-NEO PLASMID

The changes of the growth characteristics and surface ultrastructure during longterm passages of Wg3h cells that have been transfected with PSV_2-neo (Wg3h-neo) were studied. The results showed that the growth and DNA synthesis rate were evidently higher in the transfected cells than in the parental wg3h cells. The saturation of the transfected cell growth density was also increased, but neither the parental and transfected cells formed cell colony in soft agar medium nor tumor growth in nude mice. There was no significant differences in morphology between the two cell types under light microscopy, however much more microvilli were seen on the transfected cell surface under scanning electron microscopy. Southern blot hybridization analysis indicated that the PSV_2-neo plasmids were integrated into the genome of the target cells. Our results demonstrated that the transfected cells remained as nonmalignant cells although some changes of their growth characteristics and surface ultrastructure appeared.