| 体外负压培养对人BMSCs BMP -2、VEGF mRNA表达水平的影响 |
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| 引用本文： | 杨治,朱养均,何鹏,张银刚. 体外负压培养对人BMSCs BMP -2、VEGF mRNA表达水平的影响[J]. 中国矫形外科杂志, 2011, 19(17): 1476-1479 |
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| 作者姓名： | 杨治 朱养均 何鹏 张银刚 |
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| 作者单位： | 陕西中医学院附属西电医院骨科;西安交通大学医学院第一附属医院骨科; |
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| 基金项目： | 国家自然基金资助项目(编号:30973063); 中国博士后科学基金资助项目(编号:20070421123); 陕西省卫生厅科研基金(编号:2010323); 西安市卫生局科技立项(编号:J2010B31) |
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| 摘 要： | [目的]探讨体外负压培养对人骨髓间充质干细胞(bone marrow-derived stroma cells,BMSCs)骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2)、血管内皮生长因子(vascular endothelial growth factor,VEGF)mRNA表达水平的影响。[方法]取第3代BMSCs分为实验组和对照组,实验组进行间歇性负压培养,设置压力为17 kPa,30 min/次,4次/d,干预2周;对照组于普通CO2培养箱中常规培养。免疫组织化学染色检测BMP-2、VEGF的表达水平,RT-PCR检测2周后以及终止负压后1、2、3 d BMP-2、VEGF mRNA表达水平。[结果]诱导2周后,BMSCs呈现出显著的成骨细胞特性,与对照组比较,实验组细胞BMP-2、VEGF mRNA表达水平显著提高,且差异均有统计学意义(P<0.05),负压终止后3 d,两组细胞BMP-2 mRNA表达水平无明显差异(P>0.05),VEGF mRNA却在负压培养终止后的3 d内与对照组比较仍有显著差异(P<0.05)。[结论]体外负压培养可以提高BMSC...
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| 关 键 词： | 负压 BMSCs BMP-2 VEGF |
Effects of negative pressure on the mRNA expression of BMP-2 and VEGF in human BMSCs cultured in vitro |
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| YANG Zhi,ZHU Yang-jun,HE Peng,et al.. Effects of negative pressure on the mRNA expression of BMP-2 and VEGF in human BMSCs cultured in vitro[J]. Orthopedic Journal of China, 2011, 19(17): 1476-1479 |
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| Authors: | YANG Zhi ZHU Yang-jun HE Peng et al. |
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| Affiliation: | YANG Zhi,ZHU Yang-jun,HE Peng,et al.Department of Orthopaedics,the Xidian Affiliated Hospital,Shanxi Traditional Chinese Medicine,Xi'an 710077,China |
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| Abstract: | [Objective]To investigate the effects of negative pressure on the mRNA expression of bone morphogenetic protein-2(BMP-2) and vascular endothelial growth factor(VEGF) in human bone marrow-derived stroma cells(BMSCs) in vitro. [Method]The third passage cells were divided into negative pressure treatment group and control group.The treatment group was induced by negative pressure intermittently(pressure: 17 kPa,30 min/time,and four times a day)for two weeks.The control group was cultured in conventional condit... |
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| Keywords: | negative pressure BMSCs BMP-2 VEGF |
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