3TSR对胰腺癌生长及转移抑制的实验研究

目的 研究血管生成抑制因子3TSR对胰腺癌生长和转移的抑制作用. 方法 采用人胰腺腺癌细胞株MIAPa Ca-2,建立类似于临床的胰腺癌原位转移裸鼠模型.分别自腹腔注射3TSR、键择(Gemcitabine,Gem)和3TSR+Gem,注射3周后处死动物,测量原位肿瘤体积、抑瘤率,观察肿瘤细胞肝、腹膜转移及腹水情况. 结果 体内实验时3TSR组、Gem组和3TSR+Gem组肿瘤体积较对照组明显减少,抑瘤率分别为71.9%,80.1%和85.8%,但该3组间平均肿瘤体积差异无统计学意义(P>0.05);对照组、3TSR组、Gem组和3TSR+Gem组肝转移率分别为66.7%,22.2%,44.4%和11.1%;腹膜转移率分别为100%,66.7%,66.7%和22.2%;腹水发生率分别为77.8%,55.6%,22.2%和11.1%.与对照组相比,3TSR组胰腺癌肝转移率显著降低(P<0.05),而腹膜转移及腹水发生率差异无统计学意义.3TSR联合Gem后,胰腺癌的腹膜、肝转移及腹水形成均受到显著抑制(P<0.05). 结论 3TSR对体内胰腺癌生长和肝转移具有一定的抑制作用,与传统化疗药物Gem联用后对胰腺癌肝和腹膜转移均起到抑制作用,并可抑制腹水形成.

Experimental study of 3TSR on inhibitory effects on growth and metastasis of pancreatic carcinoma in nude mice

Objective To investigate the effects of 3TSR on the growth and metastasis of pancreatic carcinoma in nude mice.Methods An animal model of orthotopic metastatic pancreatic cancer was established in which the pancreatic cancer cell line MIAPa Ca-2 was inoculated into the pancreas. 3TSR,Gemcitabine (Gem) and 3TSR plus Gem were administered by intraperitoneal injection for three consecutive weeks,respectively. The volume of the primary pancreatic tumor, the number of liver and peritonium metastases, and the ascitic fluid were assessed.Results Compared with control group,the average size of the tumor decreased significantly in 3TSR, Gemcitabine (Gem) and 3TSR plus Gem groups (P<0.05),but there is no significant difference among above-mentioned three groups. The liver metastatic rate in 3TSR,Gemcitabine (Gem) and 3TSR plus Gem groups was 66.7%,22.2%,44.4% and 11.1%,respectively. The peritoneum metastatic rate was 100%,66.7%,66.7% and 22.2%,respectively. The rate of ascites formation was 77.8%,55.6%,22.2% and 11.1%,respectively. Compared with control group,3TSR significantly decreased the liver metastasis (P<0.05). Combination of 3TSR with Gem significantly decreased liver metastasis, the peritoneum metastasis and ascites formation (P<0.05).Conclusion 3TSR inhibits the growth and liver metastasis of human pancreatic carcinoma in nude mice. Combination of 3TSR with Gem can synergistically inhibit the liver and peritoneum metastasis of pancreatic carcinoma in nude mice and ascites formation.