| 干扰素γ/Fas介导的HaCaT细胞凋亡及8种人参皂甙的作用 |
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| 引用本文： | 刘影,涂彩霞,郝大程,张蕴颖,杨凌.干扰素γ/Fas介导的HaCaT细胞凋亡及8种人参皂甙的作用[J].中国皮肤性病学杂志,2007,21(10):583-585,602. |
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| 作者姓名： | 刘影 涂彩霞 郝大程 张蕴颖 杨凌 |
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| 作者单位： | 1. 大连医科大学附属第二医院皮肤科,辽宁,大连,116027
2. 中国科学院大连化学物理研究所药用资源开发组,辽宁,大连,116027 |
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| 摘 要： | 目的体外诱导角质形成细胞凋亡并研究人参皂甙对细胞凋亡的影响。方法将IFN-γ,Anti Fas Ab作用于体外培养角质形成细胞HaCaT诱导细胞凋亡,并检测人参皂甙对细胞凋亡的影响。结果50 ng/mL IFN-γ和25 ng/mL Anti Fas Ab共同作用可以诱导21.5%角质形成细胞HaCaT凋亡。8种人参皂甙中,Mx,CK和Re可逆转Anti Fas Ab诱导的HaCaT细胞凋亡,逆转率分别为18.4%,14.5%和9.6%。结论IFN-γ和Anti Fas Ab共同作用可诱导HaCaT细胞凋亡。人参皂甙Mx,CK和Re对Anti Fas Ab诱导的HaCaT细胞凋亡有一定的抑制作用。
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| 关 键 词： | 湿疹性皮炎 凋亡 人参皂甙 |
| 文章编号： | 1001-7089（2007）10-0583-03 |
| 收稿时间： | 2007-03-13 |
| 修稿时间： | 2007-03-132007-05-14 |
IFNγ/Fas Induced HaCaT Cells Apoptosis and the Effects of Ginsenosides on Apoptosis |
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| LIU Ying,TU Cai-xia,HAO Da-cheng,et al.IFNγ/Fas Induced HaCaT Cells Apoptosis and the Effects of Ginsenosides on Apoptosis[J].The Chinese Journal of Dermatovenereology,2007,21(10):583-585,602. |
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| Authors: | LIU Ying TU Cai-xia HAO Da-cheng |
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| Institution: | Department of Dermatology, the Second Affiliated Hospital, Dalian Medical University, Dalian 116027, China |
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| Abstract: | Objective Induced HaCaT cells apoptosis by INFγand Anti-Fas monoclonal antibody(Anti Fas Ab) and study potential therapeutic effect of ginsenosides.Methods The keratinocyte cell line,HaCaT,was exposed to IFN-γ and AntiFas Ab,and observed effects of ginsenosides on apoptosis.Results Flow cytometry demonstrated that IFN-γ and AntiFas Ab induced 21.5% HaCaT apoptosis.Ginsenosides Mx,CK and Re,among eight ginsenosides screened,inhibiting KC apoptosis 18.4%,14.5%,9.6% respectively.Conclusion Combination of IFN-γ and Anti Fas Ab induces the apoptosis of HaCaT cells.Ginsenosides Mx,CK,and Re inhibit the apoptosis of HaCaT cells. |
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| Keywords: | Eczematous dermatitis Apoptosis Ginsenosides |
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