| 生物素—亲和素系统检测华支睾吸虫特异性抗体的研究 |
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| 引用本文： | 汪冰 黎世涛. 生物素—亲和素系统检测华支睾吸虫特异性抗体的研究[J]. 华西医学, 1993, 8(2): 170-172 |
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| 作者姓名： | 汪冰 黎世涛 |
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| 作者单位： | 华西医科大学传染病学教研室，华西医科大学传染病学教研室，四川省寄生虫病防治研究所，四川省寄生虫病防治研究所 |
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| 摘 要： | 用ABC-ELISA检测华支睾吸虫粪检阳性病人130例,粪检阴性受试者201例及其他疾病82例、并和粪检及另外两种血清学方法(SPA ERASA、常规ELISA)比较:和粪检阳性符合率93.08％,阴性符合率63.37％;ABC-ELISA的阳性反应强度是常规ELISA的1.31倍(p<0.05),其平均几何滴度是SPA-ELISA的1.77倍(p<0.05),特别在轻度感染病人,ABC-ELISA的平均几何滴度明显高于后两种血清学方法;而且非特异性反应较低。
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| 关 键 词： | 异形吸虫病 生物素 亲和素 抗体 |
Avidin-Biotin Complex in the Detection Antibody of Clonorchis Sinensis |
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| Wang Bing,et al West China University of Medical Sciences,Chengdu. Avidin-Biotin Complex in the Detection Antibody of Clonorchis Sinensis[J]. West China Medical Journal, 1993, 8(2): 170-172 |
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| Authors: | Wang Bing et al West China University of Medical Sciences Chengdu |
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| Abstract: | Avidin-biotin complex ELISA (ABC-ELISA) in detecting the specific antibody of clonorchis sinensis was studied. By comparing with stool examination and traditional ELISA, SPA-ELISA: The sensitivity and specificity of ABC-ELISA were 93.08% and 63.37% respectivilly. The positive reaction intensity of ABC-ELISA was 1.31 times more than traditional ELISA (p<0.05). The GMRT of ABC-ELISA was 1.77 times more than SPA-ELISA (p<0.05). The GMRT of ABC-ELISA was higher than the other two immunological methods in detecting the lower level of antibody and with a low crossreaction to other diseases. |
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| Keywords: | ABC-ELISA Clonorchis sinensis. |
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