肾上腺皮质肿瘤VEGF、TSP-1的表达及其与微血管密度的关系

目的 研究血管内皮生长因子(VEGF)、血小板反应素1(TSP-1)在肾上腺皮质肿瘤中的表达及其与微血管密度(MVD)的关系.方法 采用免疫组化方法检测13例肾上腺皮质癌、30例肾上腺皮质腺瘤和7例正常肾上腺组织中VEGF、TSP-1及MVD(以CD34为标记)的表达.结果 肾上腺皮质癌VEGF表达高于肾上腺皮质腺瘤,差异有统计学意义(P＜0.01);TSP-1在正常肾上腺皮质表达明显高于肾上腺皮质癌(P＜0.01);MVD在肾上腺皮质癌为(107.67±11.91)/视野,显著高于肾上腺皮质腺瘤(59.46±21.31)/视野和正常肾上腺皮质组织(25.10±3.94)/视野(均P＜0.01);VEGF的表达与MVD呈正相关(P＜0.01),TSP-1的表达与MVD呈负相关(P＜0.01),VEGF与TSP-1的表达呈负相关(P＜0.01).结论 VEGF和TSP-1在肾上腺皮质肿瘤的表达失衡是其肿瘤血管异常生成的重要原因.

Association of VEGF,TSP-1 expression and microvessel density in adreocortical tumors

Objective To study the expressions of vascular endothelial growth factor (VEGF) and thrombospondin-1 (TSP-1) in the adrenocortical tumors,and their relationship with microvessel density (MVD).Methods The expressions of TSP-1,VEGF and MVD (evaluated by CD34) were detected by immunohistochemical staining in 13 cases with adrenocortical carcinoma,30 with adrenocortical adenoma and 7 with normal adrenal cortex.Results The expression of VEGF in adrenocortical carcinomas was significantly higher than that in adrenocortical adenomas(P＜0.01),while no VEGF expression was found in normal adrenal cortex.The expression of TSP-1 was higher in normal adrenal cortex compared with adrenocortical carcinomas (P＜0.01).MVD in adrenocortical carcinomas was higher than those in adrenocortical adenomas and normal adrenal cortex[(107.67±11.91 vs 59.46±21.31 and 25.10±3.94)/field,both P＜0.01)].There was positive correlation between VEGF expression and MVD (P ＜ 0.01),negative correlation between TSP-1 expression and MVD (P＜0.01).The expression of VEGF was negatively correlated with TSP-1 (P＜0.01).Conclusions Disequilibrium in the expressions of VEGF and TSP-1 may play an important role in the angiogenesis of adrenocortical tumors.