| 脂质体介导HBcAg基因转染人树突状细胞的实验研究 |
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| 引用本文： | 郭小芙,李军,韩亚萍,宋培新,刘源,董莉,贺奇彬,孔练花. 脂质体介导HBcAg基因转染人树突状细胞的实验研究[J]. 中国血液流变学杂志, 2006, 16(1): 23-25 |
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| 作者姓名： | 郭小芙 李军 韩亚萍 宋培新 刘源 董莉 贺奇彬 孔练花 |
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| 作者单位： | 南京医科大学第一附属医院感染病科,江苏,南京,210029 |
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| 基金项目： | 江苏省“135重点学科”基金江苏省“333工程”基金资助 |
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| 摘 要： | 目的通过脂质体介导乙肝病毒核心抗原(HBcAg)基因转染人体内专职抗原提呈的树突状细胞(DC)的方法,使HBcAg基因在树突状细胞中表达,构建树突状细胞疫苗。方法分离健康人外周血单个核细胞,在粒-巨噬细胞集落刺激因子(GM-CSF)和白细胞介素-4(IL-4)的诱导下培养DC。在培养的第5天,应用脂质体PEI介导将含有编码HBcAg 基因的质粒(pJW4303／HBc)转染入DC,再以免疫印迹技术(Western-Blotting)验证其表达产物。结果经以质粒pJW4303 为真核表达载体的HBcAg目的基因转染后的树突状细胞有效表达了HBcAg抗原。结论经该方法转染后的DC目的基因可有效表达HBcAg,为以DC为基础的免疫治疗奠定了新的基础。
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| 关 键 词： | 肝炎病毒 乙型 肝炎核心抗原 乙型 树突状细胞 脂质体 转染 |
| 文章编号： | 1009-881X(2006)01-0023-03 |
| 收稿时间： | 2006-01-20 |
| 修稿时间： | 2006-01-20 |
The Significance of Expression of Hepatitis B core Antigen in Dendritic Cells transferred by HBcAg Gene in Lipofectin |
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| GUO Xiao-fu,LI Jun,HAN Ya-ping,SONG Pei-xin,LIU Yuan,DONG Li,HE Qi-bin,KONG Lian-hua. The Significance of Expression of Hepatitis B core Antigen in Dendritic Cells transferred by HBcAg Gene in Lipofectin[J]. Chinese Journal of Hemorheology, 2006, 16(1): 23-25 |
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| Authors: | GUO Xiao-fu LI Jun HAN Ya-ping SONG Pei-xin LIU Yuan DONG Li HE Qi-bin KONG Lian-hua |
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| Affiliation: | Department of Infectious Disease,the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029,China |
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| Abstract: | Objective To increase the immune response which anti-HBVthrough the method that the plasmid vector contained HBcAg gene transfer the dendritic cells(DCs) which is the professional antigen processing cell in the human being.Methods Peripheral blood mononuclear cells(PBMC) were isolated from whole blood by settling process on HES,the adherent cells were cultured in the medium RPMI-1640 contained GM-CSF and IL-4.On day 5, lipofectin-mediated gene transfection method was used to transfected DCs.The expression of HBcAg gene was confirmed by Western-Blotting.Results The expression of HBcAg from DCs which was transferred was found by Western-Blotting.Conclusion The method maked DCs which were transferred expressed HBcAg effectly ,it provide a new kind of way for the immune treatment based on DCs. |
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| Keywords: | Hepatitis B virus Hepatitis B core antigen Dendritic cells lipofectin transfection |
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