小檗碱对小鼠全脑缺血后神经元凋亡的影响

目的观察小檗碱能否对小鼠全脑缺血后细胞凋亡产生抑制作用.方法利用改良的Pulsinelli-Brierley4血管闭塞法制成小鼠全脑缺血再灌注动物模型.TUNEL法原位检测特征性DNA片段.琼脂糖凝胶电泳技术检测DNA梯度带.结果小檗碱组与再灌流各时间段缺血组相比,海马CA1区TUNEL阳性细胞数明显减少(P<0.01).缺血组再灌注24h、48h可见典型的DNA梯度带,分别在180、360、540bp等处.小檗碱组未见特征性DNA梯度带.结论小檗碱可以减少小鼠全脑缺血后海马CA1区细胞凋亡的发生.

Effects of Berberine on neuronal apoptosis after global cerebral ischemia in mice

Objective This study was performed to examine the invlovement of apoptosis during reperfusion after transient global cerebral ischemia in mice and to investigate the effects of Berberine on it. Methods The model of transient global cerebral ischemia was made by using Pulsinelli-Brierley 4 vessels occlusion methods. Nissl stain was used to observe the morphologic of neuron in the hippocampal CA 1 subfield. Apoptotic cells were detected by TUNEL technique. DNA ladder was observed by agarode gel eletrophresis with hippocampal tissue. Results Berberine reduced evidently the TUNEL positive cells in the hippocampal CA 1 subfield. DNA ladders were detected on agarode gel eletrophoresis at 24 hours and 48 hours during reperfusion after global ischemia,there were no DNA ladders in Berberine group. Conclusion Berberine can reduce apoptosis occuring after global cerebral ischemia in mice.