甲状腺癌中p53基因248位密码子突变及其蛋白过度表达的临床意义

对５４例甲状腺癌标本分别用ＤＮＡ聚合酶链反应－限制性片段长度多态性分析（ＰＣＲ－ＲＦＬＰ）法检测ｐ５３基因第２４８位密码子的突变及免疫组化法检测ｐ５３蛋白，并用时序检验分析ｐ５３基因突变和ｐ５３蛋白表达与甲状腺癌的关系。结果发现，甲状腺癌中１５例（２７８％）有ｐ５３基因突变和３０例（５５６％）有ｐ５３蛋白过度表达，临床分期晚、分化程度低的甲状腺癌突变率较高，ｐ５３抑癌基因突变和ｐ５３蛋白阳性的病例有较高的复发率和死亡率。表明ｐ５３基因突变及其蛋白过度表达在甲状腺癌的发生和发展过程中起重要作用，是预后不良的标志。

The significance of mutations and protein expression of p53 gene codon 248 in patients with thyroid carcinoma

Fifty four cases of thyroid carcinoma(TC) were studied for p53 gene codon 248 point mutations by PCR-RFLP,and their protein expression by immunohistochemical stain. To clarify if p53 gene mutation and expressions will correlate with the prognosis. Mutations of p53 gene were found in 15 cases (27.8%) and overexpression of p53 in 30 cases (55.6%) of thyroid carcinomas. Mutations of p53 gene and overexpression of p53 protein were correlated with the histopathologic type,clinical stage,rate of recurrence and mortality of TC. These results suggest that mutation of p53 gene and p53 protein overexpression play an important role in thyroid carcinogenesis,and are indicators of poor prognosis.