甜茶护齿含片对葡糖基转移酶和细胞外水不溶性多糖的影响

目的研究甜茶护齿含片对变形链球菌葡糖基转移酶和细胞外水不溶性多糖合成的影响。方法将甜茶护齿含片磨成粉末,并采用二倍稀释法,用含1%蔗糖的胰蛋白胨-胰蛋白月示-酵母提取物(TTY)液体培养基,配制成5个浓度的实验组;并以含1%蔗糖的TTY液体培养基作为阴性对照组。加入变形链球菌菌液,厌氧培养48 h后离心,一份透析提取葡糖基转移酶,采用Somogyi法和考马斯亮蓝法分别测定还原糖和总蛋白含量,计算酶活性和比活力大小;另一份弃上清液留沉淀,采用蒽酮法测定水不溶性多糖的含量。结果随着甜茶护齿含片混悬液浓度的升高,葡糖基转移酶活性和水不溶性多糖含量逐渐降低(P<0.05),除0.313 g/50 ml浓度组外,葡糖基转移酶各实验组与对照组酶活性及比活力之间差异有统计学意义(P<0.05),各实验组与对照组水不溶性多糖含量差异有统计学意义(P<0.05)。结论甜茶护齿含片对变形链球菌葡糖基转移酶和细胞外水不溶性多糖的合成具有显著抑制作用。

Influence of Rubrus Suarissimus S.Lee Dental Care Buccal Tablets on Glucosyltransferase and Water-insoluble Glucan

Objective To study the influence of Rubrus Suarissimus S.Lee dental care buccal tablets on glucosyltransferase(GTF) and extra-cellular synthesis of water-insoluble glucan(WIG) of Streptococcus mutans(S.mutans).Methods Rubrus Suarissimus S.Lee dental care buccal tablets were lapped into powder and used in the experimental group with 5 concentrations prepared with TTY which contained with 1% glucose,while TTY culture with 1% glucose as the negative control.S.mutans was added into each group,and then centrifugation was performed after 48 hours′ anaerobical culturing.One batch was that the supernatants were collected to extract GTF,which was used to measure the content of reducing sugar and total protein by Smoyi method and Bradford method,then figured out enzyme activity and the specific activity.The other batch was that the supernatants were abandoned and the sedimentations were reserved,which was used to measure the content of WIG by the Anthrone method.Results The GTF and WIG of S.mutans reduced gradually with the increase of the concentration of Rubrus Suarissimus S.Lee dental care buccal tablets(P<0.05).Except the group of 0.313 g/50 ml,there were significant differences between each experimental group and control group in glucosyltransferase activity,specific activity and WIG(all P<0.05).Conclusion Suarissimus S.Lee dental care buccal tablets could restrain the GIF and extra-cellular synthesis of WIG of S.mutans.