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| 大鼠海马锥体神经元的急性分离方法 | |
| 引用本文： | 冯冠青,赵世刚,杨雷,贺秉军,傅志燕.大鼠海马锥体神经元的急性分离方法[J].内蒙古医学杂志,2009,41(8):912-914. |
| 作者姓名： | 冯冠青 赵世刚 杨雷 贺秉军 傅志燕 |
| 作者单位： | 1. 鄂尔多斯市中心医院神经内科,内蒙古,鄂尔多斯,017000 2. 内蒙古医学院附属医院神经内科,内蒙古,呼和浩特,010050 3. 南开大学生命科学院,天津,300071 |
| 摘 要： | 目的：建立一种适用于膜片钳技术的大鼠海马锥体神经元急性分离方法,并对其离子通道进行描述。方法：采用酶加机械分离法制备10~12 d鼠龄的大鼠海马锥体神经元,用全细胞膜片钳技术测定其生理学特性。结果：分离出的神经元形态正常,有较长突起;用膜片钳技术证实,其保存了主要的离子通道活性。结论：建立一种简单快速的适用于膜片钳技术的大鼠海马锥体神经元急性分离方法。 |
| 关 键 词： | 海马锥体神经元 急性分离 膜片钳 离子通道 |
The Method of Acute Isolation of Rat Hippocampal Pyramidal Neuron |
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| FENG Guan-qing,ZHAO Shi-gang,YANG Lei,HE Bing-jun,FU Zhi-yan.The Method of Acute Isolation of Rat Hippocampal Pyramidal Neuron[J].Inner Mongolia Medical Journal,2009,41(8):912-914. | |
| Authors: | FENG Guan-qing ZHAO Shi-gang YANG Lei HE Bing-jun FU Zhi-yan |
| Institution: | 1.Department of Neurology;The Ordos Central Hospital;Ordos 017000 China;2.Department of Neurology;The Affiliated Hospital of Inner Mongolia Medical College;Huhhot 010050 China;3.The Institute of Life Science;Nankai University;Tianjin 300071 China |
| Abstract: | Objective：To develop a modified method of acute isolation of rat hippocampal pyramidal neu- ron, and observe its ion current with patch clamp. Methods： The hippocampal pyramidal neurons were obtained by a combination of mechanical and enzymatic means from 10 -- 12 day - old rats. Whole - cell patch clamp tech- nique was used to measure the electrophysiological properties of the neurons. Results： The neurons isolated in this way had regular configuration. The neuraxons of them were very long. With patch- clamp techniques, we found that the methods could save the activities of primary iro.n channels. Conclusion： A simple and fast method for acutely isolating rats hippocampal pyramidal neuron suitable for patch clamp is successfully found. |
| Keywords: | Hippocampal pyramidal neuron Acutely isolating Patch clamp Ionic channel |
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