红景天苷对高原红细胞增多症大鼠红细胞膜结构和功能的影响

目的研究红景天苷对高原红细胞增多症(HAPC)大鼠红细胞膜结构与功能的影响,为红景天苷防治HAPC提供科学依据。方法 40只Wistar大鼠随机分为对照组、HAPC模型组及红景天苷高、中、低剂量(200、100、50 mg/kg)组,每组8只,雌雄各半。除对照组外,其余4组制备HAPC模型。对照组、模型组大鼠ig生理盐水,红景天苷组大鼠同时ig红景天苷,给药体积为10 mL/kg,1次/d,连续给药40 d。给药结束后,股动脉取血,采用生化、酶联免疫法测定大鼠红细胞膜脂质流动性和红细胞膜总胆固醇含量、总磷脂含量,以及磷脂成分磷脂酸(PA)、磷脂酰胆碱(PC)、磷脂酰乙醇胺(PE)、磷脂酰丝氨酸(PS)的含量,Na~+,K~+-ATPase和Ca~(2+),Mg~(2+)-ATPase的活性,红细胞内Na~+和Ca~(2+)浓度。结果红景天苷可显著提高HAPC大鼠红细胞膜脂质流动性和红细胞膜总磷脂、PA、PC、PE、PS含量,提高Na~+, K~+-ATPase和Ca~(2+), Mg~(2+)-ATPase的活性;显著降低红细胞膜总胆固醇含量、红细胞内Na~+和Ca~(2+)浓度。结论红景天苷可通过调控红细胞膜脂质成分,改善红细胞膜的功能和细胞代谢活动,进而缓解HAPC的相关症状。

Effects of salidroside on structure and function of erythrocyte membrane in high altitude polycythemia rats

Objective To investigate the effects of salidroside on the structure and function of erythrocyte membrane in rats with high altitude polycythemia (HAPC), and to provide the scientific basis for the mechanism of salidroside preventing and treating HAPC. Methods A total of 40 Wistar rats were randomly divided into five groups, control group, HAPC model group, salidroside high-dose (200 mg/kg), medium-dose (100 mg/kg), and low-dose (50 mg/kg) groups, four female and four male rats in each group. In addition to the control group, the remaining four groups established the HAPC model. The rats in the control group and model group were ig administered with saline, and rats in salidroside group were treated with different doses of salidroside at the same time. The dosage volume was 10 mL/kg, and once a day for 40 d. After the administration, blood was collected from femoral artery of the rats. Biochemical and enzyme-linked immunoassay were used to determine the lipid fluidity of erythrocyte membrane, total cholesterol content, total phospholipids content, the content of phospholipid components including phosphatidic acid (PA), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), the activity of Na⁺, K⁺-ATPase and Ca²⁺, Mg²⁺-ATPase on erythrocyte membrane, and the concentrations of Na⁺ and Ca²⁺ in erythrocyte. Results Salidroside can significantly improve the lipid fluidity, total phospholipids, PA, PC, PE, and PS content of erythrocyte membrane, and improve the activity of Na⁺, K⁺-ATPase and Ca²⁺, Mg²⁺-ATPase of erythrocyte membrane in HAPC rats. Total cholesterol content on erythrocyte membrane, and the concentrations of sodium and calcium in erythrocyte were significantly reduced. Conclusion These findings suggested that salidroside can improve the function of erythrocyte membrane and cell metabolic activities by regulating the lipid composition of erythrocyte membrane, thereby alleviating the symptoms associated with high altitude polycythemia.