多药耐药相关蛋白1和CD34在大肠癌组织中的表达及其意义

目的 探讨多药耐药相关蛋白1(MRP1)和小血管内皮细胞标志物CD34在大肠癌组织中的表达及其与临床病理指标的关系.方法 应用免疫组织化学法对53例大肠癌患者的癌组织、正常组织中MRP1和CD34表达进行检测,将结果与临床病理指标进行分析.结果 MRP1在大肠癌及正常组织的阳性表达率分别为49.1％和15.1％,差异有统计学意义(x2=14.029,P＜0.01).MRP1的表达与分化程度、浸润深度、淋巴结转移等病理特征均无关.CD34在大肠癌及正常组织的表达分别为(35.63±12.23) MVD/HP和(6.12 ±0.97) MVD/HP,差异有统计学意义(t=17.565,P＜0.01).CD34的表达与年龄、性别、肿瘤大小、生长部位均无关,与Dukes分期、淋巴结转移、分化程度、肠壁浸润深度均有关(均P＜0.05).结论 MRP1和CD34在大肠癌组织中高表达,可能与大肠癌的发生有关；MRP1可能与原发性多药耐药性有关,CD34可能参与了大肠癌的侵袭和转移,联合检测可为大肠癌的诊治和预后评估提供新的参考指标.

Expression of MRP1 and CD34 in colorectal carcinoma tissue and its clinical significance

Objective To explore the expression of MRP1 and CD34 in colorectal carcinoma tissue and the relationship with clinicopathological factors. Methods Immunohistochemical streptavidin-perosidase method was used to examine the expression of MRP1 and CD34 in 53 cases with colorectal carcinoma and normal colorectal tissue. The correlation between the levels of MRP1 and CD34 expression and clinicopathological factors were analyzed. Results The positive expression rates of MRP1 in the carcinoma group and normal colorectal tissue group were 49. 1% and 15.1% respectively, and there was a significant difference of the positive expression between the two groups（χ2 = 14. 029, P 〈 0. 01 ）. The expression of MRP1 had no correlation with the degree of differentiation, the depth of invasion, the metastasis of lymph node and all the other clinicopathological factors（ P 〉 0.05 ）. CD34 value in the carcinoma group and normal colorectal tissue group were （35.63 ± 12.23 ） MVD/HP and （6.12 ±0.97 ） MVD/ HP, respectively, and there was a significant difference between the two groups （t = 17. 565, P 〈 O. 01 ）. CD34 was not correlated with age, sex, tumor size,localization of the primary tumor （ P 〉 0.05 ）, but correlated with Dukes staging, lymph node metastasis, differentiation of the tumor, depth of invasion （ all P 〈 0.05 ）. Conclusion The overexpression of MRP1 and CD34 protein may involve in colorectal carcinogenesis ; MRP1 may involve in the primary multidrug re- sistance in colorectal carcinoma. ;CD34 may involve in the colorectal carcinoma invasion and metastasis. Investigating the expression of MRPI and CD34 in colorectal carcinoma simultaneously can provide new referential indexes for the treatment and prognosis of colorectal carcinoma.