黄连解毒汤体内外抗肿瘤作用的实验研究

目的：观察黄连解毒汤的体内、体外抗肿瘤作用。 方法：采用小鼠肉瘤S180和小鼠前胃癌MFC等移植性肿瘤模型进行体内实验，通过检测抑瘤率、胸腺指数、脾脏指数等指标观察黄连解毒汤的体内抑瘤作用及对免疫器官的影响。并用MTT方法检测黄连解毒汤对小鼠S180、小鼠MFC、人胃癌SGC-7901、人肝癌SMMC-7721等4种瘤株的抑制作用。结果：黄连解毒汤大、中剂量组中对S180荷瘤小鼠有抑制作用，大剂量组对MFC荷瘤小鼠有抑制作用，抑瘤率分别为48.38%、33.77%、30.74%；对S180、MFC、SGC-7901和SMMC-7721细胞均有一定的细胞毒作用, IC50分别为99.73 mg?L-1、84.47 mg?L-1、153.32 mg?L-1和102.87 mg?L-1。结论：黄连解毒汤体内对S180、MFC小鼠移植瘤有一定的抗肿瘤活性，体外对4种肿瘤细胞也具有抑制作用。

Antitumor Activities of Huanglianjiedu Decoction in Vivo and in Vitro

Objective To study the antitumor activities of Huanglianjiedu Decoction（HLJDT） in vivo and in vitro. Methods Antitumor activities were tested in mice with experimental tumor S180 and MFC in vivo, thymus index, spleen index, tumor inhibitory rate were evaluated. Cell proliferation was assayed by using methyl thiazolyl tetrazolium （MTT） method in vitro. S180, MFC, SGC-7901 and SMMC-7721 cancer cells were incubated in culture media containing HLJDT. Results HLJDT showed a Antitumor activities on S180 and MFC in vivo. All of the HLJDT in different dosage groups could highly inhibit the proliferation of 4 cancer cells. Conclusion The HLJDT could inhibit the tumor S180, MFC in mice, the drug has obvious anticancer effects against S180, MFC, SGC-7901 and SMMC-7721.