| 人乳头瘤病毒16型转换基因的序列分析 |
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| 引用本文： | 许雪梅,宋国兴,司静懿,刘世德,李昆.人乳头瘤病毒16型转换基因的序列分析[J].基础医学与临床,2000,20(5):34-36. |
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| 作者姓名： | 许雪梅 宋国兴 司静懿 刘世德 李昆 |
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| 作者单位： | 中国医学科学院,中国协和医科大学基础医学研究所生物物理室,北京,100005 |
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| 基金项目： | 国家自然科学基金!39700131 |
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| 摘 要： | 为了解中国地区宫颈癌病人中人乳头瘤病毒１６型Ｅ６Ｅ７基因结构特点，从中国山东地区宫颈癌活检组织中提取组织ＤＮＡ，经ＨＰＶ多重引物ＰＣＲ法鉴定标本中感染ＨＰＶ型别，选单纯感染ＨＰＶ１６两例标本ＤＮＡ为模板进行ＰＣＲ扩增，获得ＨＰＶ１６Ｅ６Ｅ７基因后，重组入ｐＡＬＴＥＲ－１载体，进行双向测序、分析。ＤＮＡ序列分析表明：两例标本的ＨＰＶ１６Ｅ６Ｅ７序列全长均为７７６ｂｐ，与已发表的德国标准株长度相等，两
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| 关 键 词： | 人乳头瘤病毒16例 E6E7基因 宫颈癌 序列分析 |
| 修稿时间： | 1999-09-17 |
Sequence Variation of Human Papillomavirus Type 16 E6EE7 Gene from Cervical Carcinoma Biopsies in Shandong Province |
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| XU Xuemei,SONG Guoxing,SI Jingyi,et al.Sequence Variation of Human Papillomavirus Type 16 E6EE7 Gene from Cervical Carcinoma Biopsies in Shandong Province[J].Basic Medical Sciences and Clinics,2000,20(5):34-36. |
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| Authors: | XU Xuemei SONG Guoxing SI Jingyi |
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| Abstract: | To study the structure specificity of HPV16E6E7 gene of Chinese cervical carcinoma patients in Shandong province The tissue DNA were extracted from cervical carcinoma biopsies of Shandong province and then the types of HPVs which infected in cervical carcinoma tissues were identified by HPV multiple primers PCR HPV16E6E7 gene was amplified by PCR from the cervical carcinoma tissue DNA with the infection of HPV16 type only and then cloned the E6E7 gene into pALTER-I vector After sequencing the double strand the gene was compared with the prototype E6E7 gene of HPV16 Sequencing results indicated there were sequence diversity in two cases and total of five nucleotide exchanges were detected four of these led to amino acid exchanges there are structure differences between the HPV16E6E7 of Chinese and that of the standard strain |
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| Keywords: | human papilloma virus E6E7 gene cervical carcinoma sequence |
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