Analysis of NORs and NOR-associated heterochromatin in the mussel Mytilus galloprovincialis Lmk |
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Authors: | M. J. Marti´nez-Expo´sito J. Me´ndez J. J. Pasantes |
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Affiliation: | (1) Dpto. Bioloxi´a Fundamental, Xene´tica, Universidade de Vigo, E-36200 Vigo, Spain;(2) Dpto. Bioloxi´a Celulare Molecular, Xene´tica, Universidade da Coruna, E-15071 A Coruna, Spain |
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Abstract: | The chromosomes of the mussel Mytilus galloprovincialis were analysed by means of chromomycin A3 (CMA), distamycin A/DAPI (DA/DAPI), DAPI/actinomycin D (DAPI/AMD) and chromomycin A3/distamycin A/DAPI(CDD) fluorescence banding techniques, C-banding, silver staining, N-banding and in situ hybridization with 18S+28S rDNA and telomere probes. 18S+28S rDNA clusters were located on the telomeres of two pairs of submeta/subtelocentric chromosomes. The nucleolar organizing regions (NORs) were associated with bright CMA fluorescence, dull DAPI fluorescence and C- and N-positive bands, but not all four NOR-associated heterochromatin bands showed bright CMA fluorescence in a given cell; intra- and interindividual variability was found in this character. Additional non-ribosomal C-bands did not show any differential fluorescent behaviour.This revised version was published online in November 2005 with corrections to the Cover Date. |
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Keywords: | fluorochrome staining heterochromatin in situ hybridization Mytilus galloprovincialis nucleolar organizing regions |
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