| 半固态培养基法培养鼠磨牙牙胚的实验观察 |
| |
| 引用本文： | 朱奇,樊明文,张旗,边专,陈智.半固态培养基法培养鼠磨牙牙胚的实验观察[J].口腔医学研究,2000,16(4). |
| |
| 作者姓名： | 朱奇 樊明文 张旗 边专 陈智 |
| |
| 作者单位： | 武汉大学口腔医学院,湖北,武汉,430079 |
| |
| 摘 要： | 目的 :观察体外培养的鼠磨牙牙胚发育过程的组织学变化。方法 :用 16d胎龄的昆明小鼠下颌第一磨牙牙胚置于RPMI- 16 40半固态培养基上培养 ,常规组织学观察。结果 :体外培养的牙胚可由帽状期进入钟状期 ,出现成牙本质细胞和成釉细胞的分化及前期牙本质的形成。结论 :半固态培养基法培养牙胚可保持其发育过程。
|
| 关 键 词： | 牙胚 器官培养 成牙本质细胞分化 |
Study of Developing Mouse Molar Tooth Germs in vitro |
| |
| ZHU Qi,FAN Ming-wen,ZHANG Qi,et al..Study of Developing Mouse Molar Tooth Germs in vitro[J].Journal of Oral Science Research,2000,16(4). |
| |
| Authors: | ZHU Qi FAN Ming-wen ZHANG Qi |
| |
| Institution: | ZHU Qi,FAN Ming-wen,ZHANG Qi,et al. College of Stomatology,Wuhan University,Wuhan,430079 [ |
| |
| Abstract: | Objective: To study the histological characteristic change during the development of mouse molar tooth in vitro. Methods:Tooth germs(first mandibular molars) obtained from E16d Kunming mouse embryos were cultured for 3,5,7,9,12 days on semi-solid RPMI-1640 medium supplemented with 20?S. Results: In vitro the toothgerms were able to develop into more progressive stage, some mesenchymal cells differentiated into odontoblasts and deposited predentin. Conclusion: This culture condition could maintain thedevelopment of mouse molar tooth in vitro. |
| |
| Keywords: | tooth germ organ culture odontoblast differentiatio` |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
