| Dispase在成年鼠骨骼肌干细胞纯化中的价值 |
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| 引用本文： | 姚启盛 叶章群 陈丛波 王小康 王卫民 陈立新. Dispase在成年鼠骨骼肌干细胞纯化中的价值[J]. 郧阳医学院学报, 2004, 23(6): 336-337,342,F003 |
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| 作者姓名： | 姚启盛 叶章群 陈丛波 王小康 王卫民 陈立新 |
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| 作者单位： | [1]华中科技大学同济医学院附属同济医院泌尿外科，湖北武汉430000 [2]郧阳医学院附属太和医院泌尿外科 [3]郧阳医学院附属太和医院生命科学研究所 |
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| 摘 要： | 目的寻找一种最佳的骨骼肌干细胞分离及纯化方法。方法采用成年雌性SD大鼠,分别采用二步消化法和Dispase消化法分离肌干细胞,然后用差速贴壁技术纯化肌干细胞,骨骼肌肌动蛋白(α-sarcometricactin)免疫细胞化学染色对细胞进行鉴定。结果Dipase组肌干细胞的数量明显多于二步消化法组,且细胞的生长速度也明显较后者快,而衰老速度则明显慢于后者。结论Dipase可明显减轻细胞的损伤,提高肌干细胞的数量和质量,是一种最佳的细胞分离酶。
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| 关 键 词： | 骨骼肌干细胞 dispase 纯化 培养 鼠 |
| 文章编号： | 1006-9674(2004)06-0336-03 |
| 修稿时间： | 2004-09-09 |
The value of dipase in purifying skeletal muscle stem cells |
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| YAO Qi-sheng,YE Zhang-qun,CHEN Cong-bo,et al.. The value of dipase in purifying skeletal muscle stem cells[J]. Journal of Yunyang Medical College, 2004, 23(6): 336-337,342,F003 |
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| Authors: | YAO Qi-sheng YE Zhang-qun CHEN Cong-bo et al. |
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| Abstract: | Objective To explore a best way of isolating and purifying skeletal muscle stem cells.Methods The mature female SD rat's muscle cells were isolated by two step enzyme digestion and dipase,respectively, cells were purified with preplate technique and identified by immunocytochemistry. Results Comparing with two step enzyme digestion group,The number of muscle cells and it's growth in the dispase group were obviously much more and rapid, respectively, while aging process became more slowly. Conclusion Dipase is a best cell isolation enzyme which can decrease cell injury and improve cell quantity and quality. |
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| Keywords: | skeletal muscle stem cell dispase purify culture rat |
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