| 二苯乙烯苷对糖尿病大鼠肾小球系膜细胞增殖及分泌炎症因子的影响 |
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| 引用本文： | 李彩蓉,蔡飞,黎荣,赵辛元,杨颖乔.二苯乙烯苷对糖尿病大鼠肾小球系膜细胞增殖及分泌炎症因子的影响[J].中药药理与临床,2010(2). |
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| 作者姓名： | 李彩蓉 蔡飞 黎荣 赵辛元 杨颖乔 |
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| 作者单位： | 咸宁学院医学院内科教研室;咸宁学院药学院药理教研室; |
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| 基金项目： | 湖北省教育厅科研项目(项目编号B20092802) |
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| 摘 要： | 目的:探讨二苯乙烯苷(TSG)对糖尿病肾病大鼠肾小球系膜细胞增殖及分泌炎症因子的影响。方法:分别以正常糖(NG)、高糖(HG)、糖基化终产物(AGE)及过氧化氢(H2O2)孵育大鼠肾小球系膜细胞,CCK细胞计数法检测大鼠系膜细胞的OD值,判断细胞增殖情况。比色法检测细胞培养液中的超氧化物歧化酶(SOD)和谷胱甘肽(GSH-Px)的活性、丙二醛(MDA)含量的变化。采用酶联免疫吸附实验(ELISA)检测方法检测培养细胞上清中单核细胞趋化蛋白-1(MCP-1)和细胞间粘附分子-1(ICAM-1)蛋白浓度,Western Blot检测系膜细胞中环氧化酶-2(COX-2)蛋白表达。结果:HG、AGE和H2O2能明显诱导系膜细胞增殖和氧化应激增强,而TSG可明显改善系膜细胞增殖和氧化应激状态,同时三种诱因均可使肾小球系膜细胞MCP-1、ICAM-1和COX-2蛋白分泌增加;TSG预处理后可抑制上述因素诱导的炎症因子分泌。结论:TSG可抑制糖尿病大鼠系膜细胞增殖和调控炎症因子表达,从而在防治糖尿病肾病发生发展过程中发挥重要作用。
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| 关 键 词： | 二苯乙烯苷 单核细胞趋化蛋白-1 细胞间粘附分子-1 环氧化酶 糖尿病肾病 |
Effect of tetrahydroxystilbene glucoside on the cell proliferation and inflammatory factors in rat mesangial cells |
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| Li Cairong,Cai Fei,Li Rong,Zhao Xinyuan,Yang Yinqiao.Effect of tetrahydroxystilbene glucoside on the cell proliferation and inflammatory factors in rat mesangial cells[J].Pharmacology and Clinics of Chinese Materia Medica,2010(2). |
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| Authors: | Li Cairong Cai Fei Li Rong Zhao Xinyuan Yang Yinqiao |
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| Institution: | Li Cairong,Cai Fei1,Li Rong,Zhao Xinyuan,Yang Yinqiao(Department of medical,Xianning College,Xianning 437100,1 Department of pharmacology,Xianning 37100) |
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| Abstract: | Objective:To study the effect of tetrahydroxystilbene glucoside(TSG) on rat mesangial cells (GMCs) proliferation and the expression of inflammatory factor cultured separately with normal glucose,high glucose,advanced glycosylation end products (AGE) and H2O2. Methods:Cell proliferation was assessed by CCK-8,the activity of superoxide dismutase(SOD) and the levels of malondialdehyde (MDA),glutathione (GSH-Px) in supernatant were assayed using chromatomery. The levels of monocyte chemoattractant protein-1(MCP... |
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| Keywords: | tetrahydroxystilbene glucoside(TSG) monocyte chemoattractant protein-1 (MCP-1) intercellular adhesion molecule-1 (ICAM-1) cyclooxygenase-2(COX-2 ) diabetic nephropathy(DN) |
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