|
|||||
|
|
| 以HBc颗粒为呈现载体的真核表达载体pcDNA3.1-HBcNhel的构建 | |
| 引用本文： | 陈佳玉,李景利,赵文静,王海河,李凡. 以HBc颗粒为呈现载体的真核表达载体pcDNA3.1-HBcNhel的构建[J]. 中国现代医学杂志, 2006, 16(1): 54-56 |
| 作者姓名： | 陈佳玉 李景利 赵文静 王海河 李凡 |
| 作者单位： | 1. 吉林大学基础医学院,病原生物实验室,吉林,长春,130021 2. 博泰生物技术有限公司,吉林,长春,130021 3. 北方肝胆病医院,吉林,长春,130062 |
| 摘 要： | 目的构建含乙肝核心C基因的真核表达载体pcDNA3.1-HBcNhel,探索其作为DNA疫苗载体的可能性.方法采用分子克隆技术在原核表达质粒pTrc-core的HBcAg el-loop处添加NheI酶切位点,双酶切此重组载体pTrc-coreNhel及真核表达载体pcDNA3.1,产物经纯化、T4DNA连接酶连接,连接产物转化大肠杆菌DH5α,筛选抗Amp+克隆并提取质粒进行酶切及PCR鉴定.结果成功构建了真核表达质粒pcDNA3.1-HBcNhel.结论该重组质粒可作为治疗性及预防性疫苗的候选载体,为深入研究预防性及治疗性疫苗奠定了基础. |
| 关 键 词： | DNA疫苗 免疫反应 HBc基因 |
| 文章编号： | 1005-8982(2006)01-0054-03 |
| 修稿时间： | 2005-03-11 |
Use HBc as carrier to construct expression-vector PCDNA3.1-HBcNhel |
|
| CHEN Jia-yu,LI Jing-li,ZHAO Wen-jing,WANG Hai-he,LI Fan. Use HBc as carrier to construct expression-vector PCDNA3.1-HBcNhel[J]. China Journal of Modern Medicine, 2006, 16(1): 54-56 | |
| Authors: | CHEN Jia-yu LI Jing-li ZHAO Wen-jing WANG Hai-he LI Fan |
| Abstract: | [Objective] To construct DNA vaccine vector pcDNA3.1-HBcNheI. [Methods] We usedPCR to add the NheI site to plasmid pTrc-core, then HBcNheI gene was cloned into vector pcDNA3.1, the target genes were sequenced and the plasmids were extracted. [Results] We successfully constrct the DNA vaccine vector. [Conclusion] The plasmid pcDNA3.1-HBcNheI might be able to serve as curing or preventive DNA vaccines candidate. |
| Keywords: | DNA vaccine immune response HBc gene |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |