| 楮实子生物碱的细胞毒作用研究 |
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| 引用本文： | 庞素秋,王国权,黄宝康,秦路平.楮实子生物碱的细胞毒作用研究[J].中药材,2007,30(7):826-828. |
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| 作者姓名： | 庞素秋 王国权 黄宝康 秦路平 |
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| 作者单位： | 1. 解放军第180医院药械科,福建泉州,362000
2. 第二军医大学药学院,上海,200433 |
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| 摘 要： | 目的:对楮实子总生物碱抗肿瘤细胞的活性进行探讨。方法:分别用MTT及集落形成法考察楮实子中提得的总生物碱对Hela、BEL-7402、A375、SMM1990、Saos-2细胞株的细胞毒作用。结果:当药物浓度在10μg/L以下,抑制作用不明显。随着药物浓度的升至50μg/L时,药物对肿瘤细胞生长的抑制作用较明显,MTT法测得抑制率在20%以上,集落形成法测得抑制率在30%以上。而当药物浓度达到100μg/L时,楮实子总生物碱显示出较为显著的肿瘤细胞抑制作用,两种方法的细胞生长抑制率分别在50%和60%以上。结论:楮实子中总生物碱有较强的细胞毒作用。
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| 关 键 词： | 楮实子 生物碱 MTT法 集落形成法 细胞毒作用 |
| 文章编号： | 1001-4454（2007）07-0826-03 |
| 修稿时间： | 2006-10-31 |
Cytoxic Activities of Total Alkaloids Isolated from Fructus Broussonetiae in Vitro |
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| PANG Su-qiu,WANG Guo-quan,HUANG Bao-kang,QIN Lu-ping.Cytoxic Activities of Total Alkaloids Isolated from Fructus Broussonetiae in Vitro[J].Jorunal of Chinese Medicinal Materials,2007,30(7):826-828. |
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| Authors: | PANG Su-qiu WANG Guo-quan HUANG Bao-kang QIN Lu-ping |
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| Institution: | 1. Department of Pharmacy, 180^th Hospital of PLA, Quanzhou 362000, China; 2. College of Pharmacy,Second Military Medical University, Shanghai 200433, China |
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| Abstract: | Objective: To observe the cytoxic activities of total alkaloids isolated from Fructus Broussonetiae on the growth inhibition in five carcinoma cell lines.Methods: The growth inhibition was analyzed by MTT,Cell colony in the Hela,BEL-7402,A375,SMM1990,Saos-2 cell lines.Results: The growth of all tumor cells were inhibited by total alkaloids isolated from Fructus Broussonetiae.Conclusion: The results show that total alkaloids isolated from Fructus Broussonetiae possess the role of antitumors in cell culture. |
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| Keywords: | Fructus Broussonetiae Alkaloids MTT method Cell colony Cytoxic activities |
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