黄芪丹参复方成分对妊高征大鼠模型胎盘滋养细胞诱导型一氧化氮合酶mRNA表达影响研究

目的研究黄芪丹参复方成分提高胎盘血供的分子机制,为临床有效防治胎盘血供不足所致妊娠并发症提供思路。方法提取分离有效中药黄芪、丹参复方成分,运用NOS(一氧化氮合酶)阻滞剂L-精氨酸甲酯(L-NAME)制作一氧化氮合成阻滞大鼠模型,原位杂交(ISH)法,观察胎盘滋养细胞诱导型一氧化氮合酶(iNOS)mRNA表达变化及黄芪丹参复方有效成分的影响作用。结果模型组iNOS mRNA表达明显增加,与黄芪丹参治疗组、阳性药物硝酸甘油组均有显著差异(P<0.01)。结论黄芪丹参复方成分可能降低母胎界面妊娠免疫反应,阻抑胎盘免疫病理损伤,提高胎盘滋养细胞合成与释入NO进入胎盘循环,从而维持妊娠母胎循环张力,达到提高子宫-胎盘-胎儿血液供应目的。

Effect of Components of Mongolian Milkvetch Root and Dan - shen Root Compound on Induced Nitrogen Oxide Synthesis mRNA Expression in Placental Trophocyte of Pregnancy- induced Hypertension Syndrome Rat Model

Objective To find new effective prevention and treatment of pregnant complications induced by placental blood supply insufficiency,the molecular mechanism of the components of Mongolian milkvetch root-Dan-shen root compound that enhanced placental blood supply was studied.Methods The effective components of Mongolian milkvetch root-Dan-shen root were extracted,nitrogen oxide synthesis blocking model was established by administration of L-Arginine methyl ester(L-NAME) to pregnant rats;and the change of the expression of iNOS mRNA in placental trophocyte was assessed by in situ hybridization to evaluate the effect of the active components of milkvetch-red sage compound. Results The expression of iNOS mRNA of model group was obviously enhanced significantly as compared with both the Mongolian milkvetch root-Dan-shen root treatment and the positive control nitroglycerin treatment groups(P<0.01).Conclusion The components of Mongolian milkvetch root-Dan-shen root compound can restrain the immune reaction of placenta,restrain the placental immune pathologic damage through the improvement of NO synthesis and release by placental trophocyte into the placental circulation,thus to keep the circulative tension between mother and fetus and to increase blood supply among uterus-placenta-fetus.