| HPLC法测定三七止血胶囊中三七总皂苷含量研究 |
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| 引用本文： | 肖遐,阳国平.HPLC法测定三七止血胶囊中三七总皂苷含量研究[J].湖南师范大学学报(医学版),2012,9(1):91-96. |
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| 作者姓名： | 肖遐 阳国平 |
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| 作者单位： | 1. 中南大学湘雅三医院,湖南长沙410013;湖南师范大学医学院,湖南长沙410013
2. 中南大学湘雅三医院,湖南长沙,410013 |
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| 摘 要： | 目的:完善三七止血胶囊的质量标准,控制药品质量。方法:采用高效液相色谱法测定三七止血胶囊中三七皂苷R1﹑人参皂苷Rg1﹑人参皂苷Rb1的含量。色谱柱:色谱柱:C18Alltima 250×4.6 mm,5μL;流动相:以流动相A:乙腈,流动相B:水,采用梯度洗脱方式测定,检测波长:203nm;流速:1.0mL/min。结果:测定10批样品,三七皂苷的平均含量为3.04 mg/粒,三七皂苷R1线性范围为0.1008～0.9072μg,相关线性系数:R=0.9999,人参皂苷Rg1线性范围为0.4160～3.7440μg,相关线性系数:R=0.9999,人参皂苷Rb1线性范围为0.4136～3.7224μg。相关线性系数:R=0.9999,平均回收率:94.41%,RSD:0.74%(n=5)。结论:建立的方法简便易行,重现性好,可有效的控制该制剂的质量。
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| 关 键 词： | 三七止血胶囊 三七皂苷 人参皂苷 质量标准 高效液相色谱法 |
Determination of Saponins Content in panax notoginseng capsule With HPLC |
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| Xiao Xia
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Yang Guoping.Determination of Saponins Content in panax notoginseng capsule With HPLC[J].Journal of Hunan Normal University(Medical Science),2012,9(1):91-96. |
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| Authors: | Xiao Xia Yang Guoping |
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| Institution: | 1(1.The third Xiangya Hospital,Changsha 410013,China;2.Medical College,Hunan Normal University,Changsha 410013,China) |
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| Abstract: | Objective To perfect the quality panax notoginseng and to control its quality.Methods Determination of the contents of ginsenoside Rg1,Rb1 and the notoginsenoside R1 in the total notoginsenosides of the Panax notogineseng by HPLC,Column:separated on C18 column（250 × 4.6mm Alltima,5 μL） using a gradient mobile phase consisting of acetonitrile-water buffer.Results Determination of 10 batches of samples,the average recovery was 3.04 mg / tablets,ginsenoside Rg1 showed a good linear correlation in the range of 0.4160 to 3.7440μg（R=0.9999）,the average recovery rate was 94.41%,and RSD 0.74%（n=5） Conclusion The method is simple,feasible and reproducible this study provides a effective method for controling of the quality of preparation. |
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| Keywords: | panax notoginseng capsule notoginsenoside ginsenoside Quality control standard HPLC |
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