加巴喷丁对癫痫持续状态大鼠海马区神经元凋亡的影响

目的研究加巴喷丁(GBP)对癫痫持续状态(SE)大鼠海马区神经元凋亡的影响。方法SD大鼠48只随机分为对照组、戊四氮致痫组(PTZ组)、GBP组、GBP干预组(干预组),每组12只。戊四氮溶液60 mg.kg-1.d-1腹腔注射诱发SE。干预组致痫后给予GBP 600 mg.kg-1.d-1;GBP组仅给予GBP 600 mg.kg-1.d-1;对照组和PTZ组给予0.9%氯化钠溶液灌胃。采用头皮针状电极单极导联法观察大鼠痫性放电的脑电图。缺口末端标记法(TUNEL)检验凋亡阳性细胞。结果 TUNEL阳性细胞在PTZ组显著高于对照组(P<0.01);GBP组与对照组比较差异无统计学意义(P>0.05);干预组表达低于PTZ组(P<0.05)。结论戊四氮致SE大鼠海马CA1和CA3区神经元TUNEL阳性细胞显著增加,GBP能显著减少TUNEL阳性细胞。

Effects of gabapentin on apoptotic neurons in hippocampus of rats with status epilepticus

Objective To investigate effects of gabapentin(GBP) on apoptotic neurons in hippocampus of rats with status epilepticus(SE).Methods Forty-eight SD rats were randomly divided into 4 groups(12 rats in each group):control group,PTZ group,GBP group(600mg·kg-1·d-1),GBP treatment group(600mg·kg-1·d-1).The animal models with SE were induced by intraperitoneal injection with pentrolone(PTZ) solution(60mg·kg-1·d-1) in PTZ group and GBP intervention groups.The rats in GBP intervention group and GBP group were given GBP(600mg·kg-1·d-1) by gavage.The rats in PTZ group and the control group were given sodium chloride solution by gavage.The electroencephalogram(EEG) of epileptic discharge was observed by scalp heedle electrodes unipolar lead method.The apoptosis rate of neurons was detected by TdT-mediated dUTP nick end labeling(TUNEL).Results The numbers of TUNEL-positive neurons in PTZ group were significantly higher than those in control group(P<0.01),but there were no significant differences between GBP group and control group(P>0.05).The numbers of TUNEL-positive neurons in GBP intervention group were significantly lower than those in PTZ group(P<0.05).Conclusion PTZ increases the numbers of TUNEL-positive neurons in hippocampal CA1 and CA3 regions of SE rats,however,GBP can significantly decrease the numbers of TUNEL-positive neurons in SE rats.