不同浓度碱性成纤维细胞生长因子对体外培养兔肌腱细胞增殖的影响

目的探讨不同浓度的碱性成纤维细胞生长因子(bFGF)对体外培养兔肌腱细胞增殖的影响,并确定促进肌腱细胞增殖的最佳浓度。方法在体外培养第2代兔肌腱细胞的培养液中分别加入不同浓度(0.5、1、2、5、10、20、30、40、50μg/L)的bFGF,继续培养48 h,噻唑蓝(MTT)法染色,在酶联免疫检测仪上测出不同浓度bFGF组光密度(OD)值。结果与对照组OD均值相比:5、10、20、30、40、50μg/L bFGF组差异均有统计学意义(P<0.05);各组OD均值随bFGF浓度的增加,先逐渐增大(5～20μg/L),达到峰值后又逐渐降低(20～50μg/L)。结论 bFGF有明显促肌腱细胞增殖的作用,且与浓度有一定相关性,即促肌腱细胞增殖的起始bFGF浓度为5μg/L,而20μg/L时可达到促肌腱细胞增殖的最佳浓度。

Effect of basic fibroblast growth factor in different concentration on proliferation of tendon cells cultured in vitro

Objective To study the effects of basic fibroblast growth factor(bFGF) in different concentration on proliferation of tendon cells cultured in vitro and to estimate the best bFGF concentration to promote proliferation of tendon cells cultured in vitro.Methods The second generated tendon cell was cultured in vitro with different concentrations(0.5,1,2,5,10,20,30,40,50 μg/L) of bFGF for 48 hours,then detected by MTT methods and their optical density(OD) average values were detected by enzyme linked immunodetection meter.Results Compared to control group: 5,10,20,30,40,50 μg/L bFGF groups had significant difference(P<0.05);When bFGF concentration varying from 5 μg/L to 20 μg/L,the OD average values increase gradually,while varying from 20 μg/L to 50 μg/L,it decreased step by step.Conclusions bFGF can significant promote the proliferation of tendon cells cultured in vitro depending on its proper concentration;5 μg/L bFGF is the initial concentration and 20 μg/L is the best concentration in promoting proliferation of tendon cells cultured in vitro.