海参糖胺聚糖对内皮细胞vWF和PAI-1表达的影响

目的探讨玉足海参糖胺聚糖（GAG）对活化人脐静脉内皮细胞（HUVECs）von Willebrand因子（vWF）和纤溶酶原激活剂抑制物-1（PAI-1）表达的影响，以进一步明确GAG抗血栓作用机制。方法不同浓度GAG与凝血酶活化HUVECs共同孵育，MTT比色法检测GAG对细胞活力的影响，分别采用ELISA法和RQ—PCR法检测GAG对活化HUVECs vWF和PAI-1抗原蛋白和基因表达的影响。结果GAG可以降低活化HUVECsvWF抗原和PAI-1抗原的表达，5mg／LGAG抑制作用最为显著（P〈0．001），且强于相同剂量的肝素。5mg／LGAG还可以抑制活化HUVECsvWFmRNA和PAI-1 mRNA转录（P〈0.01、0.05），5mg／L肝素虽然对此两种mRNA转录亦呈抑制作用，但对PAI-1mRNA转录的抑制作用无统计学意义（P〉0．05）。结论GAG可能通过降低活化HUVECsvWF和PAI-1抗原表达及其mRNA转录，从而达到抗凝血及抗血栓作用。

Effects of sea cucumber glycosaminoglycan on expression of vWF and PAI-1 in stimulated human umbilical vein endothelial cells

Objective To investigate the effect of glycosaminoglycan (GAG) extracted from sea cucumber on expression of von Willebrand factor (vWF) and plasminogen activator inhibitor-1(PAI-1) in thrombin-stimulated human umbilical vein endothelial cells (HUVECs) to further explore its antithrombotic mechanisms. Methods HUVECs were pre-induced with thrombin and then co-cultured with GAG,the viability of cells was determine by MTT assay. The protein and mRNA expression of vWF and PAI-1 was detected by ELISA and real-tim...