三聚氰胺对SD雌鼠卵巢毒性的研究

目的:初步探讨不同剂量三聚氰胺对SD雌鼠卵巢的毒性作用。方法:将60只SD雌性大鼠随机分A、B、C、D四组,A、B、C为实验组,D为对照组。分别予以400 mg/kg、800 mg/kg、1 600 mg/kg的三聚氰胺灌胃,1次/日,持续35天,用药结束后24 h处死大鼠。切取卵巢称量湿重、做病理切片、记数各级卵泡。取大鼠卵巢组织固定切片,做HE染色;用免疫组化方法检测P450arom在大鼠卵巢中的分布及表达。结果:A、B、C 3组大鼠卵巢湿重与D组相比明显减轻(P<0.05);B、C组卵巢湿重相比无统计学差异,但与A组相比亦有减轻(P<0.05);A、B、C 3组的总卵泡数、成熟卵泡数、初级卵泡数和生长卵泡数明显少于D组(P<0.05)。与对照组比较,各实验组P450表达强度减弱(P<0.05);B组和C组各级卵泡数均减少,B组伴间质及颗粒细胞的崩解坏死;A组生长卵泡、成熟卵泡数均明显减少。结论:三聚氰胺所致的卵巢毒性与摄入量有一定的相关性。过量的三聚氰胺能够抑制鼠体内细胞色素芳香化酶P450的表达,使体内E2生成障碍,从而影响卵泡发育。

Study on toxicity of melamine to ovaries of female SD rats

Objective: To preliminarily explore the toxicity of different doses of melamine(MEL) to ovaries of female SD rats.Methods: Sixty female SD rats were randomly divided into 4 groups.The rats in the four groups were treated with 0,400,800,and 1 600 mg / kg MEL,respectively,once a day for successive 35 days.All the rats were sacrificed at 24 hours after the last infusion.The ovarian smears of rats were observed under light-microscope after HE staining.The distribution and expression of P450arom in the ovaries were examined by immunohistochemical assay.Results: The wet weights of ovaries in group A,group B,and group C were lighter than that in group D(P < 0.05),there was no difference between group B and group C,but the wet weights of ovaries in group B and group C were lighter than that in group A(P < 0.05).The numbers of total follicles,mature follicles,primary follicles,and growth follicles in experimental groups were less than those in group D(P < 0.05);compared with control group,the expression of P450 weakened(P < 0.05);the numbers of follicles at all levels in group B and group C decreased;the numbers of growth and mature follicles in group A decreased.Conclusion: The ovarian toxicity induced by MEL is related to dosage.High-dose MEL can inhibit the expression of P450,reduce produce of estradiol,and affect follicular development.