| NaHS抑制宫颈癌细胞增殖的初步机制 |
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| 引用本文： | 盛顺良,黄余良,周建斌,何平平,张涛.NaHS抑制宫颈癌细胞增殖的初步机制[J].中国妇幼保健,2012,27(33):5375-5377. |
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| 作者姓名： | 盛顺良 黄余良 周建斌 何平平 张涛 |
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| 作者单位： | 南华大学附属第二医院妇产科,湖南 衡阳,421001 |
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| 基金项目： | 中国博士点基金新教师类联合项目〔20114324120004〕;湖南省教育厅项目〔11C1094〕 |
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| 摘 要： | 目的:探讨NaHS对子宫颈癌细胞增殖的影响及其机制。方法:首先将子宫颈癌HeLa细胞分成对照组和不同剂量的NaHS处理组,并采用MTT法检测细胞存活率和流式细胞仪检测细胞凋亡率。使用不同浓度的格列本脲预先处理HeLa细胞30 min,再用400μmol/L的NaHS处理24 h,探讨NaHS抑制子宫颈癌细胞增殖的初步机制。结果:不同剂量的NaHS处理组能明显抑制HeLa细胞增殖而促进HeLa细胞凋亡;格列本脲预先处理可以部分阻断这种作用。结论:NaHS抑制子宫颈癌细胞增殖可能与ATP敏感性钾通道有关。
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| 关 键 词： | NaHS 子宫颈癌 增殖 格列本脲 |
The preliminary mechanism of NaHS for inhibiting cell proliferation of cervical cancer |
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| Institution: | SHENG Shun-Liang,HUANG Yu-Liang,ZHOU Jian-Bin et al.Department of Gynecology and Obstetrics,the Second Affiliated Hospital of Nanhua University,Hengyang 421001,Hu’nan,China |
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| Abstract: | Objective:To explore the effect of NaHS on cell proliferation of cervical cancer and the mechanism.Methods:Cervical cancer HeLa cells were divided into control group and NaHS treatment groups of different doses,MTT method was used to detect the survival rate and flow cytometry was used to detect apoptosis rate.Glibenclamide of different concentrations was used to pretreat HeLa cells for 30 minutes,then 400 μmol/L NaHS was used to treat HeLa cells for 20 hours,the preliminary mechanism of HaHS for inhibiting cell proliferation of cervical cancer was analyzed.Results:HaHS of different doses inhibited proliferation of HeLa cells obviously and promote apoptosis of HeLa cells;pretreatment with glibenclamide could block the effect.Conclusion:The inhibiting effect of NaHS for cell proliferation of cervical cancer may be correlated with ATP sensitive potassium channels. |
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| Keywords: | NaHS Cervical cancer Proliferation Glibenclamide |
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