不明原因复发性流产患者血清及母胎界面中IL-2、IL-10的表达及意义

目的:研究不明原因复发性流产患者的血清、蜕膜和绒毛组织中IL-2、IL-10的表达水平及意义,探讨其免疫学机制,为其早期诊断及治疗提供理论依据。方法:用酶联免疫法测定血清中的IL-2、IL-10含量,同时应用免疫组织化学染色方法检测IL-2、IL-10在绒毛和蜕膜组织中的局部表达。结果:不明原因复发性流产组血清中IL-2的浓度为(12.31±2.64)pg/ml明显高于正常早孕组的(5.74±1.22)pg/ml,差异有统计学意义(P<0.05);血清中IL-10的浓度为(7.97±3.02)pg/ml与正常早孕组的(17.6±5.12)pg/ml比较差异有统计学意义(P<0.05);血清中IL-10/IL-2比值为(0.71±0.39)与正常早孕组的比值(3.37±1.49)比较差异有统计学意义(P<0.05)。血清中两种细胞因子浓度与复发性流产组中不同流产次数之间差异无统计学意义(P>0.05);IL-2在复发性流产组蜕膜细胞胞浆和绒毛滋养叶细胞中的表达均明显高于正常早孕组(P<0.05),IL-10在复发性流产组蜕膜细胞胞浆和绒毛滋养叶细胞中的表达均低于正常早孕组(P<0.05)。结论:IL-10有利于妊娠的顺利进行,而IL-2增多可能对妊娠有害。Th1/Th2细胞平衡向IL-2偏移时可能导致流产。

Expressions and significances of IL - 2 and IL - 10 in serum and maternal - fetal interface in women with unexplained recurrent spontaneous abortion

Objective:To study the expression levels and significances of interleukin-2(IL-2) and IL-10 in serum,decidua,and villi of patients with unexplained recurrent spontaneous abortion(URSA),explore the immunological mechanism,and provide theoretical basis for early diagnosis and treatment of RSA.Methods:ELISA was used to detect the contents of serum IL-2 and IL-10,and the expression levels of IL-2 and IL-10 in villi and decidua were detected by immunohistochemical staining.Results:The serum concentration of IL-2 in URSA group was(12.31±2.64) pg/ml,which was statistically significantly higher than that in normal early pregnancy group (5.74±1.22) pg/ml](P<0.05).There was statistically significant difference in serum concentration of IL-10 between URSA group (7.97± 3.02) pg/ml] and normal early pregnancy group (17.6±5.12) pg/ml](P<0.05).There was statistically significant difference in ratio of serum IL-10/IL-2 between URSA group(0.71±0.39) and normal early pregnancy group(3.37±1.49)(P<0.05).There was no correlation between serum levels of IL-10 and IL-2 and the times of abortion in URSA group(P>0.05).The expression levels of IL-2 in cytoplasm in decidual cells and villous trophoblast cells in URSA group were statistically significantly higher than those in normal early pregnancy group(P<0.05),while the expression levels of IL-10 in cytoplasm in decidual cells and villous trophoblast cells in URSA group were statistically significantly lower than those in normal early pregnancy group(P<0.05).Conclusion:IL-10 is conducive to the smooth progress of pregnancy,while the increase of IL-2 may be harmful to pregnancy.The balance of Th1/Th2 drifting to IL-2 may lead to abortion.